Mouse ASGPR Monoclonal Antibody | anti-ASGPR antibody
ASGPR, Rat, mAb 8D7
Reactivity
Reactivity: Rat. Cross reactivity: Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Synonyms
ASGPR; Monoclonal Antibody; Rat; mAb 8D7; ASGPR 1; ASGP-R 1; Hepatic lectin 1; MHL-1; anti-ASGPR antibody
Host
Mouse
Reactivity
Reactivity: Rat. Cross reactivity: Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-ASGPR antibody
F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Application Notes: W: A non-reduced sample treatment and SDS-Page was used. The band size is 42 kDa (ref.4)
Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Immunogen
Crude rat liver membrane extracts
Positive Control
Rat hepatocytes
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-ASGPR antibody
Product Description: The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor- revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.
References
1. Mizuno, M et al: Development of a monoclonal antibody identifying an antigen which- is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Japan 1986, 21: 238 2. Mizuno, M et al;- Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251
3. Hyodo, I et al;- Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80
4. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55
5. Hirai, M et al. Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor- antibody. Acta med okayama 2002 56: 135
6. Keyel, P et al. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol boil Cell 2006 17:4300
7. Zhou, X et al. Effects of hepatitis B virus S protein on human sperm function. Hum Reprod 2009 1: 1
3. Hyodo, I et al;- Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80
4. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55
5. Hirai, M et al. Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor- antibody. Acta med okayama 2002 56: 135
6. Keyel, P et al. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol boil Cell 2006 17:4300
7. Zhou, X et al. Effects of hepatitis B virus S protein on human sperm function. Hum Reprod 2009 1: 1
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Product Notes
The ASGPR (Catalog #AAA584392) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ASGPR, Rat, mAb 8D7 reacts with Reactivity: Rat. Cross reactivity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASGPR can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunofluorescence (IF), Western Blot (WB). Application Notes: W: A non-reduced sample treatment and SDS-Page was used. The band size is 42 kDa (ref.4) Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Researchers should empirically determine the suitability of the ASGPR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASGPR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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