Mouse EF-Tu Monoclonal Antibody | anti-EF-Tu antibody
EF-Tu, E.coli, mAb 900
Applications
Immunofluorescence, Western Blot
Synonyms
EF-Tu; Monoclonal Antibody; E.coli; mAb 900; EF-TU; Elongation factor TUF; Elongation factor Tu; anti-EF-Tu antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Sequence Length
363
Applicable Applications for anti-EF-Tu antibody
Immunoassay (IA), Immunofluorescence (IF), Western Blot (W)
Application Notes
Application Use: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen
E. coli K-12 C600 Rif (pKT146) bacteria
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-EF-Tu antibody
Product Description: The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins. Monoclonal antibody 900 recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).
References
1. Weber, S et al; An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains. J Bacteriol 1995, 177: 11 2. Baensch, M et al; Conservation of the amino-terminal epitope of elongation factor Tu in eubacteria and archaea. Microbiology 1998, 144: 2241
3. Kunert, A et al; Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein. J Immunol 2007, 179: 2979
3. Kunert, A et al; Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein. J Immunol 2007, 179: 2979
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
39,642 Da
NCBI Official Full Name
EF-Tu, partial (chloroplast)
UniProt Protein Name
Elongation factor Tu, chloroplastic
UniProt Gene Name
tufA
UniProt Synonym Gene Names
EF-Tu
UniProt Entry Name
EFTU_GYMST
Uniprot Description
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
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Product Notes
The EF-Tu tufa (Catalog #AAA584299) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EF-Tu can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA), Immunofluorescence (IF), Western Blot (W). Application Use: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the EF-Tu tufa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EF-Tu, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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