Mouse anti-Human Punctin-1 Monoclonal Antibody
Punctin-1, Human, mAb 1B2-8-14
Reactivity
Human
Applications
Western Blot
Synonyms
Punctin-1; Monoclonal Antibody; Human; mAb 1B2-8-14; anti-Punctin-1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b
Form/Format
1 ml (100 u/ml) 0.2 um filtered antibody solution in PBS containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-Punctin-1 antibody
Immunoassay (IA), Western Blot (WB)
Application Notes
Application Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Punctin-1 antibody
Product Description: Monoclonal antibody 1B2-8-14 reacts with Punctin-1 (ADAMTSL-1). Punctin-1 is a secreted molecule resembling members of the family of proteases designated ADAMTS (a disintegrin-like and metalloprotease domain with thrombospondin type I motif). ADAMTS proteases are secreted enzymes that consist of a metalloprotease domain (which provides the catalytic activity) and ancillary domains (which provide substrate, cell or extracellular matrix activity). Punctin-1 lacks the pro-metalloprotease and the disintegrin-like domain typical of this family but contains other ADAMTS domains in precise order including 4 thrombospondin-I repeats. Punctin-1 is an extracellular matrix protein in tissues and deposited into the cell substratum and localized towards the periphery of transfected cells. Punctin-1 and Punctin-2 (ADAMTSL-3) are the first members of a novel family of ADAMTS-like proteins that appear to have important functions in the extracellular matrix.
Monoclonal antibody 1B2-8-14 recognizes the spacer domain and reacts with molecular species of 55 kDa or a doublet of 55 kDa and 60 kDa (glycosylated form). Note that proteolysis of Punctin-1 has been described and additional smaller bands may also be obtained.
Monoclonal antibody 1B2-8-14 recognizes the spacer domain and reacts with molecular species of 55 kDa or a doublet of 55 kDa and 60 kDa (glycosylated form). Note that proteolysis of Punctin-1 has been described and additional smaller bands may also be obtained.
References
Hirohata, S et al; Punctin, a novel ADAMTS-like molecule, ADAMTSL-1, in extracellular matrix. J Biol Chem 2002, 277: 12182
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Product Notes
The Punctin-1 (Catalog #AAA584211) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Punctin-1, Human, mAb 1B2-8-14 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Punctin-1 can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA), Western Blot (WB). Application Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. Researchers should empirically determine the suitability of the Punctin-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Punctin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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