Human RNP/Sm IgG ELISA Kit | RNP/Sm IgG elisa kit
RNP/Sm IgG ELISA Kit
Reactivity
Human
Synonyms
RNP/Sm IgG; RNP/Sm IgG ELISA Kit; RNP/Sm IgG ELISA; RNP/Sm IgG elisa kit
Reactivity
Human
Samples
Human Serum Or Plasma (Citrate Plasma, Na-Heparin Plasma, Edta Plasma)
Preparation and Storage
Product should be stored at 2-8 degree C. Product is stable for 24 months from the date of manufacturing.
Related Product Information for RNP/Sm IgG elisa kit
Intended Uses: Rheumatoid Factor Total is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG, IgM and IgA class rheumatoid factor antibodies in human serum or plasma (Citrate plasma, Na-heparin plasma, EDTA plasma).
Principle of the Assay: Fc fragments of highly purified human Immunoglobulin G are bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG, anti-human IgM and anti-human IgA immunologically detect the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG, IgM and IgA antibodies present in the original sample.
Principle of the Assay: Fc fragments of highly purified human Immunoglobulin G are bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG, anti-human IgM and anti-human IgA immunologically detect the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG, IgM and IgA antibodies present in the original sample.
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Product Notes
The Human RNP/Sm IgG (Catalog #AAA580082) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA580082 ELISA Kit recognizes Human RNP/Sm IgG. It is sometimes possible for the material contained within the vial of "RNP/Sm IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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