Rabbit Isocitrate dehydrogenase Polyclonal Antibody | anti-ICDH antibody
Rabbit anti Porcine Isocitrate dehydrogenase, conjugated with Biotin
Gene Names
ICDH; F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
Reactivity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Dot Blot, Immunoblot, Western Blot
Purity
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Synonyms
Isocitrate dehydrogenase; Polyclonal Antibody; Rabbit anti Porcine Isocitrate dehydrogenase; conjugated with Biotin; anti-ICDH antibody
Host
Rabbit
Reactivity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Clonality
Polyclonal
Specificity
Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to isocitRate dehydrogenase from porcine heart.
Purity/Purification
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Form/Format
Biotin-coupled hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
Applicable Applications for anti-ICDH antibody
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
Application Notes
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as ELISA and Western blotting and immunofluorescence or histochemical techniques).
Direction for Use: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/15,000.
Direction for Use: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/15,000.
Conjugation
Biotin
Preservative
No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Immunogen
Isocitrate dehydrogenase isolated and purified from porcine heart. Freund's complete adjuvant is used in the first step of the immunization procedure.
Physicochemical Characteristics
IgG protein concentration 10mg/ml. Biotin/IgG protein molar ration (B/P) approximately 6.5. No foreign proteins added.
Marker
N-Hydroxysuccinimidobiotin.
Conjugation Procedure
A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step, activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
Preparation and Storage
Lyophilized at 4 degree C for at least 10 years. Reconstituted at or below -20 degree C for 3-5 years. Reconstituted at 4 degree C for 7 days.
Product Categories/Family for anti-ICDH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,234 Da
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Symbol
ICDH
NCBI Official Synonym Symbols
F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
NCBI Protein Information
isocitrate dehydrogenase
UniProt Protein Name
Peroxisomal isocitrate dehydrogenase [NADP]
Protein Family
UniProt Gene Name
ICDH
UniProt Entry Name
ICDHX_ARATH
NCBI Description
NADP-specific isocitrate dehydrogenase (ICDH)
Uniprot Description
Function: May be involved in response to oxidative stresses
By similarity.
Catalytic activity: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor: Binds 1 magnesium or manganese ion per subunit
By similarity.
Subcellular location: Peroxisome Ref.5 Ref.6.
Sequence similarities: Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Research Articles on ICDH
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Product Notes
The ICDH icdh (Catalog #AAA573099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Porcine Isocitrate dehydrogenase, conjugated with Biotin reacts with Cross-reactivities against enzymes of other sources may occur but have not been determined. and may cross-react with other species as described in the data sheet. AAA Biotech's Isocitrate dehydrogenase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB). The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as ELISA and Western blotting and immunofluorescence or histochemical techniques). Direction for Use: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/15,000. Researchers should empirically determine the suitability of the ICDH icdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Isocitrate dehydrogenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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