Sheep anti Human IgG (Fc specific) Secondary Antibody
Sheep anti Human IgG (Fc specific), conjugated with TRITC
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Synonyms
Sheep anti Human IgG (Fc specific); Polyclonal Secondary Antibody; conjugated with TRITC; Sheep anti Human IgG (Fc specific) secondary antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Specificity
Tetramethylrhodamine isothiocyanate-conjugated F(ab')2 fragments of polyclonal Sheep IgG antibodies to Human IgG, Fc specific.
Applicable Applications for Sheep anti Human IgG (Fc specific) secondary antibody
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
Source
Purified IgG prepared from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product
TRITC-coupled sheep F(ab’)2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Reactivity Note
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this conjugate has not been tested in detail.
Preparation and Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Related Product Information for Sheep anti Human IgG (Fc specific) secondary antibody
The antiserum is reacting with to the Fc subunits of IgG. It does not react with other immunoglobulins or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Product Categories/Family for Sheep anti Human IgG (Fc specific) secondary antibody
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Product Notes
The Sheep anti Human IgG (Fc specific) (Catalog #AAA571344) is a Secondary Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Sheep anti Human IgG (Fc specific), conjugated with TRITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sheep anti Human IgG (Fc specific) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF). Researchers should empirically determine the suitability of the Sheep anti Human IgG (Fc specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sheep anti Human IgG (Fc specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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