IgG Negative Control (FITC) and IgG Negative Control (PE) Negative Control

COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE)

Cat. Num. AAA570400

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: IgG Negative Control (FITC) and IgG Negative Control (PE); COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE); IgG Negative Control (FITC) and IgG Negative Control (PE) negative control

• Reactivity: Mouse
• Isotype: IgG1
• Clone Number: VI-AP; VI-AP
• Specificity: VI-AP reacts with calf intestine alkaline phosphatase and does not show cross-reactivity with human proteins.
• Form/Format: PBS pH 7.2, 1% BSA, 0.05% NaN3

• Applicable Applications for IgG Negative Control (FITC) and IgG Negative Control (PE) negative control: Flow Cytometry (FC/FACS)
• Application Notes: Permeabilization and Staining Procedure; - In combination with our Permeabilization Kit FIX&PERM intracellular isotype controls can be easily stained in cell suspensions.; - For each sample to be analyzed add 50 ul of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube; - Add 100 ul of Reagent A (Fixation Medium, stored and used at room temperature); - Incubate for 15 minutes at room temperature; - Add 5 ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g; - Remove supernatant and add to cell pellet 100 ul Reagent B (Permeabilization Medium) and 20 ul of COMBI-IC Negative Control antibody conjugate.; - Vortex at low speed for 1-2 seconds; - Incubate for 15 minutes at room temperature; - Wash cells with phosphate buffered saline as described above; - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2-8 degree C in the dark. Analyze; fixed cells within 24 hours.
• CE Mark: CE
• Conjugation: FITC and PE
• Preparation and Storage: Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

Related Product Information for IgG Negative Control (FITC) and IgG Negative Control (PE) negative control

This ready to use Negative Control reagent contains a combination of fluorescein and phycoerythrin conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human intracellular or cell surface antigens, same low range of nonspecific binding to human leukocytes as other COMBI-IC-Reagents. Like all other COMBI-IC-reagents, this reagent should be used in combination with our FIX&PERM Cell Permeabilization Kit (Cat.No. GAS-002)

These isotype control IgG1 are suitable as negative controls to be used in combination with COMBI-IC reagents for the:

- Enumeration of Myeloid Cells
- Analysis of Myeloid Differentiation Stage
- Enumeration of B-cells and Precursors
- Enumeration of T-cells and Precursors
- Analysis of Leukemia Cells
- Analysis of Immunodeficiency States

Results must be put within the context of other tests as well as the clinical history of the patient by a certified professional before final interpretation.

References

J. L. Clarke, W. Watkins, J Biol Chem 271, 10317 (1996).
R. N. Knibbs et al., J Cell Biol 133, 911 (1996).
B. Kniep et al., J Biochem (Tokyo) 119, 456 (1996).
A. J. Wagers, L. M. Stoolman, R. Kannagi, R. Craig, G. S. Kansas, J Immunol 159, 1917 (1997).
M. Noguchi, N. Sato, H. Sugimori, K. Mori, K. Oshimi, Leuk Res 25, 847 (2001).
W. M. Watkins, J. L. Clarke, Adv Exp Med Biol 491, 231 (2001).