Mouse anti-Human Lysozyme Antibody | anti-LYZ antibody

Mouse anti Lysozyme, conugated to PE

Cat. Num. AAA570361

• Gene Names: LYZ; LZM
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: Lysozyme; Mouse anti Lysozyme; conugated to PE; anti-LYZ antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: LZ-2
• Specificity: The anti-Lysozyme Antibody (clone LZ-2) reacts with intracellular human lysozyme/muramidase expressed by virtually all myelomonocytic cells, macrophages and their precursors.; ; The sensitivity of LZ-2 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells).In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
• Form/Format: Purified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3
• Sequence Length: 148

• Applicable Applications for anti-LYZ antibody: Flow Cytometry (FC/FACS)
• Application Notes: Permeabilization and Staining Procedure; - In combination with our Permeabilization Kit FIX&PERM intracellular Lysozyme can be easily stained in cell suspensions.; - For each sample to be analyzed add 50 ul of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube; - Add 100 ul of Reagent A (Fixation Medium, stored and used at room temperature); - Incubate for 15 minutes at room temperature; - Add 5ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g; - Remove supernatant and add to cell pellet 100 ul Reagent B (Permeabilization Medium) and 20 ul of the LZ-2 monoclonal antibody conjugate; - Vortex at low speed for 1-2 seconds; - Incubate for 15 minutes at room temperature; - Wash cells with phosphate buffered saline as described above; - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2- 8 degree C in the dark. Analyze; fixed cells within 24 hours.
• CE Mark: CE
• Conjugation: PE
• Preparation and Storage: Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

Related Product Information for anti-LYZ antibody

Lysozyme (LZ) is a cationic antimicrobial peptide of 14 kDa. Lysozyme is stored in primary but predominantly in specific (secondary) granules of neutrophils. It cleaves peptidoglycan constituents of the bacterial cell wall and can bind LPS. The epitope recognized by antibody LZ-2 is expressed by virtually all myeloid cells including normal and malignant granulocytes and monocytes. In normal myelopoiesis LZ can first be detected at the myeloblast stage where it appears somewhat later than MPO expression.

The LZ-2 antibody permits the identification and enumeration of human myelomonocytic cells using flow cytometry. Results must be put within the context of other tests as well as the clinical history of the patient by a certified professional before final interpretation. Analysis performed with this antibody should be paralleled by positive and negative controls.

References

Braylan, R. C., Orfao, A., Borowitz, M. J. & Davis, B. H. (2001) Cytometry 46, 23-7.
Catovsky, D., Matutes, E., Buccheri, V., Shetty, V., Hanslip, J., Yoshida, N. & Morilla, R. (1991) Ann Hematol 62, 16-21.
Cowland, J. B. & Borregaard, N. (1999) J Leukoc Biol 66, 989-95.
Groeneveld, K., te Marvelde, J. G., van den Beemd, M. W., Hooijkaas, H. & van Dongen, J. J. (1996) Leukemia 10, 1383-9.
Konikova, E., Glasova, M., Kusenda, J. & Babusikova, O. (1998) Neoplasma 45, 282-91.
Lanza, F., Latorraca, A., Moretti, S., Castagnari, B., Ferrari, L. & Castoldi, G. (1997) Cytometry 30, 134-44.
Oehler, L., Majdic, O., Pickl, W. F., Stockl, J., Riedl, E., Drach, J., Rappersberger, K., Geissler, K. & Knapp, W. (1998) J Exp Med 187, 1019-28.
Paietta, E. (2003) Best Pract Res Clin Haematol 16, 671-83. Strobl, H. & Knapp, W. (2004) J Biol Regul Homeost Agents 18, 335-9.

NCBI and Uniprot Product Information

• NCBI GI #: 307142
• NCBI GeneID: 4069
• Molecular Weight: 16,537 Da
• NCBI Official Full Name: lysozyme (EC 3.2.1.17)
• NCBI Official Synonym Full Names: lysozyme
• NCBI Official Symbol: LYZ
• NCBI Official Synonym Symbols: LZM
• NCBI Protein Information: lysozyme C; c-type lysozyme; 1,4-beta-N-acetylmuramidase C
• UniProt Protein Name: Lysozyme C
• Protein Family: Lysozyme
• UniProt Gene Name: LYZ
• UniProt Synonym Gene Names: LZM
• UniProt Entry Name: LYSC_HUMAN

NCBI Description

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

Uniprot Description

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family.

Protein type: Secreted, signal peptide; Secreted; EC 3.2.1.17; Endoplasmic reticulum; Vesicle; Hydrolase

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; lysozyme activity

Biological Process: retinal homeostasis; metabolic process; cytolysis; defense response to bacterium; inflammatory response

Disease: Amyloidosis, Familial Visceral