UbcH5b active protein

UbcH5b (human), (recombinant) (His-tag)

Cat. Num. AAA566725

• Gene Names: UBE2D2; UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2
• Purity: >=90% (SDS-PAGE)
• Synonyms: UbcH5b; UbcH5b (human); (recombinant) (His-tag); UbcH5b active protein

• Host: E Coli
• Purity/Purification: >=90% (SDS-PAGE)
• Form/Format: Liquid. In 25mM TRIS-HCl, pH7.5, containing 150mM NaCl and 0.5mM DTT

• Biological Activity: The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The [C85A] mutation completely abolishes the ability of the enzyme to form a thiol ester and thereby acts as an excellent negative control
• Preparation and Storage: For long term storage, store at -80 degree C; Enzyme is stable to multiple freeze/thaw cycles; Shipping: Shipped on Dry Ice

Related Product Information for UbcH5b active protein

A number of E2s in Saccharomyces cerevisiae and their homologues have been identified. One such family of E2s is the UBC4/5, characterised as essential for the degradation of short-lived, regulatory and abnormal proteins. Protein levels of S. cerevisiae UbC4/5 are up-regulated in response to stress, and their loss results in severe effects on cellular functions.

A human gene product that is 79% identical to S. cerevisiae UBC4/5 in amino-acid sequence was identified as UbcH5a. In addition, two other human members of this highly conserved E2 class were also cloned and designated as UbcH5b and UbcH5c, having 88% and 89% identity to UbcH5a, respectively.

Members of the UbcH5a/b/c are the most active class of E2s in cell extracts. The importance of this enzyme class is underscored by the critical role of UBC4/5 in S. cerevisiae. UbcH5a stimulates the conjugation of ubiquitin to the tumour-repressor p53 in the presence of E6-AP and E6. Moreover, UbcH5 family is implicated in c-fos recognition, the modulation of which is controlled by the ubiquitin-proteasome pathway. UbcH5b and UbcH5c are associated with the signal-induced conjugation and subsequent degradation of  IkBalpha in the presence of the SCF complexes. UbcH5c also catalyses the ubiquitination leading to the processing of p105 precursor to form p50, a subunit of the heterodimeric transcription factor NF-kB. The range and diversity of substrates and E3s with which this class of E2 enzymes interact, suggest their complex roles in cellular functions require to be studied further.

Product Categories/Family for UbcH5b active protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4507775
• NCBI GeneID: 7322
• NCBI Accession #: NP_003330.1
• NCBI GenBank Nucleotide #: NM_003339.2
• UniProt Accession #: P62837; P51669; Q3MN78; Q96RP6; D3DQC9
• Molecular Weight: 16,735 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 D2 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2D 2
• NCBI Official Symbol: UBE2D2
• NCBI Official Synonym Symbols: UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 D2; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2-17 kDa 2; p53-regulated ubiquitin-conjugating enzyme 1; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 D2
• UniProt Gene Name: UBE2D2
• UniProt Synonym Gene Names: PUBC1; UBC4; UBC5B; UBCH4; UBCH5B
• UniProt Entry Name: UB2D2_HUMAN

NCBI Description

Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

UBE2D2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: nucleoplasm; protein complex; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein polyubiquitination; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; protein modification process; protein ubiquitination; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway