MMP-10 active protein

MMP-10 (catalytic domain) (human), (recombinant)

Cat. Num. AAA566620

• Gene Names: MMP10; SL-2; STMY2
• Purity: Partially purified by single-step affinity chromatography and gel filtration
• Synonyms: MMP-10; MMP-10 (catalytic domain) (human); (recombinant); MMP-10 active protein

• Host: E Coli
• Purity/Purification: Partially purified by single-step affinity chromatography and gel filtration
• Form/Format: Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20uM ZnCl2, 0.5% Brij-35, and 30% glycerol

• Source Note: Active Matrix Metalloproteinase-10 (MMP-10, stromelysin-2, transin-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-10 (Phe99-Glu271, NM_2425) with a C-terminal purification tag. This comprises an active form of MMP-10 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected
• Activity: Preincubation of MMP-10 catalytic domain at 22nM with the broad-spectrum inhibitor GM6001 at 100nM for 1 hour inhibits enzymatic activity by 95%
• Specific Activity: >=18U/ug. One U=100 pmol/min at 37 degree C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100uM) as substrate
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Product Categories/Family for MMP-10 active protein

Cellular Analysis; Cell Organelles & Structures; Proteostasis; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• NCBI GI #: 4505205
• NCBI GeneID: 4319
• NCBI Accession #: NP_002416.1
• NCBI GenBank Nucleotide #: NM_002425.2
• UniProt Accession #: P09238; Q53HH9; B2R9X9
• Molecular Weight: 54,151 Da
• NCBI Official Full Name: stromelysin-2 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 10 (stromelysin 2)
• NCBI Official Symbol: MMP10
• NCBI Official Synonym Symbols: SL-2; STMY2
• NCBI Protein Information: stromelysin-2; MMP-10; transin 2; transin-2; stromelysin 2; matrix metalloprotease 10; matrix metalloproteinase-10; matrix metalloproteinase 10 (stromelysin 2)
• UniProt Protein Name: Stromelysin-2
• UniProt Gene Name: MMP10
• UniProt Synonym Gene Names: STMY2; SL-2; MMP-10
• UniProt Entry Name: MMP10_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP10: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. Belongs to the peptidase M10A family.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; EC 3.4.24.22; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis; regulation of cell migration