Flagellin [FliC] Active Protein
Flagellin [FliC] (high purity)
Reactivity
Human, Mouse
Purity
>=95% (SDS-PAGE)
Synonyms
Flagellin [FliC]; Flagellin [FliC] (high purity); Flagellin [FliC] active protein
Reactivity
Human, Mouse
Specificity
Binds to human and mouse TLR5 (Toll-like receptor 5)
Purity/Purification
>=95% (SDS-PAGE)
Form/Format
Lyophilized. Contains PBS
Concentration
0.1mg/ml after reconstitution (varies by lot)
Source Note
Isolated from Salmonella typhimurium strain 14028
Endotoxin
<0.05EU/ug purified protein (LAL test
Biological Activity
Tests positive for activation of HEK-Blue hTLR5 reporter cell line
Reconstitution
Reconstitute with 100ul sterile water
Preparation and Storage
Stable for at least 6 months after receipt when stored at -20 degree C
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20 degree C
Shipping: Shipped on Blue Ice
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20 degree C
Shipping: Shipped on Blue Ice
Related Product Information for Flagellin [FliC] active protein
Motile bacteria produce flagella, filaments composed mainly of the structural subunit flagellin. Flagellins are highly conserved molecules among both gram negative and gram positive bacteria, especially in 170 N-terminal and 100 C-terminal amino acids. In mammalian cells, Toll-like receptor 5 (TLR5) senses various flagellins resulting in activation of innate and adaptive immunity. The phase 1 flagellin FliC from Salmonella typhimurium (serotype H 'I'), a protein of 494 amino acids, upregulates production of chemokines and antimicrobial compounds in human epithelial cells and monocytes and induces maturation of dendritic cells. Historical results have shown that flagellin tests positive for activation of HEK-Blue hTLR5 reporter cell line (Invivogen) at concentrations >1 pg/ml.
Product Categories/Family for Flagellin [FliC] active protein
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The Flagellin [FliC] (Catalog #AAA566222) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The Flagellin [FliC] (high purity) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Flagellin [FliC], Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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