Rabbit anti-Human Tau Polyclonal Antibody | anti-Microtubule-associated protein Tau antibody

Tau (human) polyclonal antibody (TAUY9)

Cat. Num. AAA565271

• Gene Names: MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Synonyms: Tau; Polyclonal Antibody; Tau (human) polyclonal antibody (TAUY9); anti-Microtubule-associated protein Tau antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human microtubule-associated protein tau.
• Form/Format: Liquid. In PBS, pH 7.2, containing 0.1% sodium azide.
• Sequence Length: 758

• Applicable Applications for anti-Microtubule-associated protein Tau antibody: Immunohistochemistry (IHC) Formalin, Western Blot (WB)
• Application Notes: Immunohistochemistry (formalin-fixed tissue section, use of high temperature antigen retrieval technique may be required); Western Blot (1:3000); Suggested dilutions/conditions may not be available for all applications.
• Immunogen: Synthetic peptide corresponding to aa 12-27 (E12DHAGTpYGLGDRKDQG27) of human tau isoform 6 (2N4R) phosphorylated at Tyr18.
• Preparation and Storage: Long term: -20 degree C; Store unopened vial at -20 degree C until required for use. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of human tau (2N4R) using various antisera. Lane 1: MW markers; Lanes 2 and 3: Tau1 (control); Lanes 2, 4 and 6: unphosphorylated tau; lanes 3, 5 and 7: p56lck phosphorylated tau; ~400ng tau per lane. Lanes 4 and 5: antiphosphotyrosine (4G10) (1:1000); Lanes 6 and 7: antiserum TA 3119 (1:3000). Enhanced chemiluminescence (1 min exposure). Image courtesy of Professor Brian Anderton (Institute of Psychiatry, London, U.K.))

Related Product Information for anti-Microtubule-associated protein Tau antibody

Tau is the principal microtubule-associated protein located in axons in the nervous system where it participates in events including the elongation, stabilisation and bundling of microtubules. The tau family consists of six isoforms identified by the insertion of three or four partial repeats of 30-31 amino acids in the C-terminal region. Subcellular fractionation studies have revealed tau to be associated with the membrane fraction, as well as with the cytoskeletal fraction and there is some evidence that the N-terminal domain of tau may be responsible for membrane binding as well as interaction with other cellular components. Tau extracted from human fetal and neonatal brains is moderately phosphorylated with 5-9 phosphorylated serine/threonine residues per molecule. Normal adult human brain tau shows a marked reduction in phosphorylation, but in certain neurodegenerative diseases (tauopathies) tau becomes hyperphosphorylated (6-15 phosphates per tau molecule have been identified) and associates to form filamentous aggregates. In Alzheimer's disease (AD) hyperphosphorylated tau polymerises to form a mixture of paired helical filaments (PHF) and straight filaments which accumulate and contribute to the formation of neurofibrillary tangles - one of the characteristic features of AD. It was reported in 1998 that tau could be tyrosine phosphorylated when co-transfected with the src-family protein tyrosine kinase fyn. In 2004 the same group, using phospho-specific antibodies, reported that fyn phosphorylated Tyr18 of brain tau at an early developmental stage in mice, but that tyrosine phosphorylated tau was undetectable in adult mice. However, they also showed that PHF preparations were reactive to the tau-pTyr18-specific antibodies, implicating a role for fyn - and possibly other tyrosine kinases - in the neurodegenerative process.

Product Categories/Family for anti-Microtubule-associated protein Tau antibody

Protein Synthesis & Folding; Cell Organelles & Structures

NCBI and Uniprot Product Information

• NCBI GI #: 334302961
• NCBI GeneID: 4137
• UniProt Accession #: P10636; P18518; Q14799; Q15549; Q15550; Q15551; Q1RMF6; Q53YB1; Q5CZI7; Q5XWF0; Q6QT54; Q9UDJ3
• Molecular Weight: 80,941 Da
• NCBI Official Full Name: Microtubule-associated protein tau
• NCBI Official Synonym Full Names: microtubule associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Tautomerase
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: MAPTL; MTBT1; TAU; PHF-tau
• UniProt Entry Name: TAU_HUMAN

NCBI Description

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: axon; axoneme; cytosol; dendrite; growth cone; microtubule; microtubule associated complex; plasma membrane; postsynaptic density; tubulin complex

Molecular Function: apolipoprotein binding; enzyme binding; microtubule binding; protein binding; protein kinase binding; protein phosphatase 2A binding; SH3 domain binding; structural constituent of cytoskeleton

Biological Process: adult walking behavior; apoptosis; axon cargo transport; axon extension; brain development; cell structure disassembly during apoptosis; female pregnancy; generation of neurons; induction of apoptosis by oxidative stress; microtubule cytoskeleton organization and biogenesis; mitochondrion transport along microtubule; negative regulation of intracellular transport; neurite development; neuron migration; positive regulation of axon extension; positive regulation of microtubule polymerization; programmed cell death; regulation of autophagy; regulation of microtubule polymerization; response to nutrient; response to organic substance

Disease: Frontotemporal Dementia; Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related; Parkinson Disease, Late-onset; Parkinson-dementia Syndrome; Pick Disease Of Brain; Supranuclear Palsy, Progressive, 1