Testing Data
(Figure 1: Detection of human LRRK2 by PAb to LRRK2 (human) (AT106) . Method: Cell extracts of HEK 293T transfected with an expression vector encoding either for DYKDDDDK-tagged human LRRK2 (lane 1) or DYKDDDDK-tagged human LRRK2DeltaN (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer, the nitrocellulose membrane was probed with PAb to LRRK2 at 1:1'000 for 1 hour at room temperature and then incubated with a goat anti-rabbit horseradish peroxidase (dilution at 1:4'000). )
Rabbit anti-Human LRRK2 Polyclonal Antibody | anti-LRRK2 antibody
LRRK2 (human) polyclonal antibody (AT106)
Immunoprecipitation (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Short term: 4 degree C
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.
Testing Data
(Figure 1: Detection of human LRRK2 by PAb to LRRK2 (human) (AT106) . Method: Cell extracts of HEK 293T transfected with an expression vector encoding either for DYKDDDDK-tagged human LRRK2 (lane 1) or DYKDDDDK-tagged human LRRK2DeltaN (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer, the nitrocellulose membrane was probed with PAb to LRRK2 at 1:1'000 for 1 hour at room temperature and then incubated with a goat anti-rabbit horseradish peroxidase (dilution at 1:4'000). )
Testing Data
(Figure 1: Detection of human LRRK2 by PAb to LRRK2 (human) (AT106) . Method: Cell extracts of HEK 293T transfected with an expression vector encoding either for DYKDDDDK-tagged human LRRK2 (lane 1) or DYKDDDDK-tagged human LRRK2DeltaN (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer, the nitrocellulose membrane was probed with PAb to LRRK2 at 1:1'000 for 1 hour at room temperature and then incubated with a goat anti-rabbit horseradish peroxidase (dilution at 1:4'000). )
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]
Uniprot Description
LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.
Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; LRRK family
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: axon; caveola; cell junction; cell soma; cytoplasm; cytoplasmic vesicle; cytosol; dendrite; dendrite cytoplasm; endoplasmic reticulum; endosome; extracellular space; Golgi apparatus; Golgi-associated vesicle; growth cone; inclusion body; intracellular; lysosome; microvillus; mitochondrial inner membrane; mitochondrial matrix; mitochondrial membrane; mitochondrial outer membrane; mitochondrion; neuron projection; perikaryon; plasma membrane; ribonucleoprotein complex; synaptic vesicle membrane; terminal button; trans-Golgi network
Molecular Function: actin binding; ATP binding; clathrin binding; glycoprotein binding; GTP binding; GTP-dependent protein kinase activity; GTPase activator activity; GTPase activity; identical protein binding; kinase activity; MAP kinase kinase activity; microtubule binding; protein binding; protein homodimerization activity; protein kinase A binding; protein kinase activity; protein serine/threonine kinase activity; receptor signaling complex scaffold activity; Rho GTPase binding; SNARE binding; syntaxin-1 binding; tubulin binding
Biological Process: activation of MAPK activity; activation of MAPKK activity; autophagy; calcium-mediated signaling; cellular response to starvation; determination of adult life span; endocytosis; Golgi organization and biogenesis; GTP metabolic process; intracellular distribution of mitochondria; lysosome organization and biogenesis; MAPKKK cascade; mitochondrion localization; mitochondrion organization and biogenesis; negative regulation of macroautophagy; negative regulation of protein amino acid phosphorylation; negative regulation of protein binding; neurite morphogenesis; neuromuscular junction development; olfactory bulb development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of autophagy; positive regulation of dopamine receptor signaling pathway; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of programmed cell death; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of protein ubiquitination; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of autophagy; regulation of dopamine receptor signaling pathway; regulation of excitatory postsynaptic membrane potential; regulation of locomotion; regulation of membrane potential; regulation of mitochondrial depolarization; regulation of neuron maturation; regulation of synaptic transmission, glutamatergic; response to oxidative stress; small GTPase mediated signal transduction; tangential migration from the subventricular zone to the olfactory bulb; Wnt receptor signaling pathway through beta-catenin
Disease: Parkinson Disease 8, Autosomal Dominant
Research Articles on LRRK2
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Product Notes
The LRRK2 lrrk2 (Catalog #AAA565220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LRRK2 (human) polyclonal antibody (AT106) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LRRK2 can be used in a range of immunoassay formats including, but not limited to, Immmunohistochemistry (IHC) (PS), Immunoprecipitation (IP), Western Blot (WB). Immunohistochemistry (paraffin sections, 1:500) Immunoprecipitation (1:100) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Researchers should empirically determine the suitability of the LRRK2 lrrk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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