Western Blot (WB)
(Western blot analysis of AIB1 using MCF-7 and T-47D cell extracts. Lane 1: Molecular Weight Markers, Lane 2: MCF-7, Lane 3: T-47D.)
Mouse anti-Human AIB1 Monoclonal Antibody | anti-AIB1 antibody
AIB1 monoclonal antibody (50B6)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~160kDa by Western blot.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of AIB1 using MCF-7 and T-47D cell extracts. Lane 1: Molecular Weight Markers, Lane 2: MCF-7, Lane 3: T-47D.)
Western Blot (WB)
(Western blot analysis of AIB1 using MCF-7 and T-47D cell extracts. Lane 1: Molecular Weight Markers, Lane 2: MCF-7, Lane 3: T-47D.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]
Uniprot Description
SRC-3: a histone acetyltransferase and nuclear receptor coactivator. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Activity is enhanced by various hormones and TNF. Acetylation by CREBBP may suppress coactivation of target genes by disrupting its association with nuclear receptors. Frequently amplified or overexpressed in breast and ovarian cancers. Five alternatively spliced isoforms have been described.
Protein type: Nuclear receptor co-regulator; Acetyltransferase; Transcription, coactivator/corepressor; EC 2.3.1.48; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20q12
Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus
Molecular Function: androgen receptor binding; chromatin binding; histone acetyltransferase activity; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein binding; protein dimerization activity; protein N-terminus binding; thyroid hormone receptor binding; transcription coactivator activity
Biological Process: androgen receptor signaling pathway; histone acetylation; multicellular organism growth; positive regulation of keratinocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter; vagina development
Research Articles on AIB1
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Product Notes
The AIB1 ncoa3 (Catalog #AAA565058) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AIB1 monoclonal antibody (50B6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AIB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (1ug/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~160kDa by Western blot. Researchers should empirically determine the suitability of the AIB1 ncoa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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