Human CRP ELISA Kit | CRP elisa kit

Human CRP ELISA Kit

Cat. Num. AAA564038

• Gene Names: CRP; PTX1; MGC88244; MGC149895
• Reactivity: Human
• Synonyms: CRP; Human CRP ELISA Kit; Human CRP; CRP elisa kit

• Reactivity: Human

• Samples: Immunoperoxidase Assay for Determination of C-Reactive Protein in Human Samples
• Assay Type: Sandwich
• Intended Uses: The CRP test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in Human biological samples.
• Preparation and Storage: Store at 4 degree C.

Related Product Information for CRP elisa kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 19301. This C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer. It is synthesized in the liver and, in humans, is normally present as a trace constituent of serum at levels less that 0.3 mG/dL. The levels in serum rise quickly following acute tissue damage and also falls very rapidly once the stimulus is removed. It has been proposed that the function of CRP is to aid in complement activation, influence phagocytic cell function, and augment cell mediated cytotoxicity. Investigations over the past few years have shown that quantification of these in plasma or serum can provide valuable information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well2.

Product Categories/Family for CRP elisa kit

ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 17975775
• NCBI GeneID: 1401
• UniProt Accession #: P02741; Q08AK3; Q8WW75; A8K078; D3DVD9; D3DVE0; Q5VVP7
• Molecular Weight: 25,039 Da
• NCBI Official Full Name: CRP
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1; MGC88244; MGC149895
• NCBI Protein Information: C-reactive protein; pentraxin 1; OTTHUMP00000033307
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq]

Uniprot Description

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: extracellular space; growth cone; extracellular region; filopodium

Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; low-density lipoprotein binding; virion binding; calcium ion binding

Biological Process: protein polymerization; wound healing; defense response to Gram-positive bacterium; response to ethanol; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging