Typical Testing Data/Standard Curve (for reference only)
Dog Osteopontin ELISA Kit | SPP1 elisa kit
Dog Osteopontin ELISA Kit
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Dog
Synonyms
Osteopontin; Dog Osteopontin ELISA Kit; SPP1 elisa kit
Reactivity
Dog
Specificity
Two retrospective studies with 398 and 138 stool specimens were performed to compare the Norovirus ELISA with another commercially available ELISA.
Sequence Length
300
Samples
Stool Specimens
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SPP1 elisa kit
Intended Uses: The Norovirus ELISA is an enzyme immunoassay for the direct detection of Norovirus specific antigens in stool specimens. For Research Use Only..
Principle of the Assay: Norovirus antigens from stool specimens and the positive control react with polyclonal anti-Norovirus antibodies coated on the solid phase of the microplate. After incubation non-bound material is removed by a washing step. Subsequently bound antigens specifically react with horseradish peroxidase (HRP) labeled polyclonal anti-Norovirus antibodies during a second incubation period. Unbound conjugate is removed by a washing step. HRP converts the subsequently added colourless chromogenic substrate solution (TMB / H2O2) into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells turning the solution from blue to yellow. The optical density (OD) of the solution read at 450 / >= 620 nm is directly proportional to the specifically bound amount of Norovirus antigens. By means of the calculated cut-off value, results are interpreted either as positive or negative!!Background/Introduction: Noroviruses belong to the family Caliciviridae, single stranded RNA viruses of 30-40 nm in size characterized by a typical cup-shaped capsid. Within the genus Norovirus the two human pathogenic genogroups GGI and GGII have been identified. GGI and GGII strains can be further subclassified into at least 15 and 18 genotypes resp. The genetic heterogeneity of Noroviruses causes distinct capsid protein divergences between different genogroups (about 60%) as well as between different genotypes within one genogroup (about 20-30%). Since 1994 genotype GGII.4 is predominantly circulating. Noroviruses are very resistant to environmental conditions and highly contagious. The infection is transmitted by direct contact to already infected people either by faecal-oral transmission or by ingestion of aerosols from vomit or by contaminated food, drinking water or objects. After a short, 10-50 hours lasting incubation time fulminant diarrhea and often vomiting develop as the characteristic symptoms. The infection is usually self-limiting and symptoms disappear after 2-3 days. Norovirus infections are characterized by seasonal fluctuations with a climax during the winter months. They are considered as the most common cause of non-bacterial gastroenteritis outbreaks worldwide, but may also be responsible for single cases of viral gastroenteritis. The high sequence variability of the capsid proteins circumvents the production of protective antibodies and hampers detection. Methods like PCR (usually as "Real time Reverse Transcriptase PCR-Rt RT-PCR) and enzyme immunoassay are commonly used for laboratory detection.
Principle of the Assay: Norovirus antigens from stool specimens and the positive control react with polyclonal anti-Norovirus antibodies coated on the solid phase of the microplate. After incubation non-bound material is removed by a washing step. Subsequently bound antigens specifically react with horseradish peroxidase (HRP) labeled polyclonal anti-Norovirus antibodies during a second incubation period. Unbound conjugate is removed by a washing step. HRP converts the subsequently added colourless chromogenic substrate solution (TMB / H2O2) into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells turning the solution from blue to yellow. The optical density (OD) of the solution read at 450 / >= 620 nm is directly proportional to the specifically bound amount of Norovirus antigens. By means of the calculated cut-off value, results are interpreted either as positive or negative!!Background/Introduction: Noroviruses belong to the family Caliciviridae, single stranded RNA viruses of 30-40 nm in size characterized by a typical cup-shaped capsid. Within the genus Norovirus the two human pathogenic genogroups GGI and GGII have been identified. GGI and GGII strains can be further subclassified into at least 15 and 18 genotypes resp. The genetic heterogeneity of Noroviruses causes distinct capsid protein divergences between different genogroups (about 60%) as well as between different genotypes within one genogroup (about 20-30%). Since 1994 genotype GGII.4 is predominantly circulating. Noroviruses are very resistant to environmental conditions and highly contagious. The infection is transmitted by direct contact to already infected people either by faecal-oral transmission or by ingestion of aerosols from vomit or by contaminated food, drinking water or objects. After a short, 10-50 hours lasting incubation time fulminant diarrhea and often vomiting develop as the characteristic symptoms. The infection is usually self-limiting and symptoms disappear after 2-3 days. Norovirus infections are characterized by seasonal fluctuations with a climax during the winter months. They are considered as the most common cause of non-bacterial gastroenteritis outbreaks worldwide, but may also be responsible for single cases of viral gastroenteritis. The high sequence variability of the capsid proteins circumvents the production of protective antibodies and hampers detection. Methods like PCR (usually as "Real time Reverse Transcriptase PCR-Rt RT-PCR) and enzyme immunoassay are commonly used for laboratory detection.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
osteopontin
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin
Protein Family
NCBI Description
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Research Articles on SPP1
Similar Products
Product Notes
The Dog SPP1 (Catalog #AAA560907) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA560907 ELISA Kit recognizes Dog SPP1. It is sometimes possible for the material contained within the vial of "Osteopontin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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