GDF2 active protein
GDF2
Gene Names
GDF2; BMP9; HHT5; BMP-9
Applications
SDS-Page
Purity
>95%, as determined by SDS-PAGE and HPLC.
Synonyms
GDF2; GDF2 active protein
Host
CHO
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Form/Format
Lyophilized; PBS, pH 7.2
Sequence
SAGAGSHCQK TSLRVNFEDI GWDSWIIAPKE YEAYECKGGC FFPLADDVTPTK HAIVQTLVHL KFPTKVGKAC CVPTKLSPIS VLYKDDMGVP TLKYHYEGMS VAECGCR
Sequence Length
429
Applicable Applications for GDF2 active protein
SDS-PAGE
Application Notes
Recombinant GDF2 migrates as an approximately 12 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Species
Human
Source Note
Optimized DNA sequence encoding Human GDF2 mature chain was expressed in CHO. Recombinant Human Growth/differentiation factor 2 encodes amino acid residues 320-429.
Biological Activity
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,320 Da
NCBI Official Full Name
growth/differentiation factor 2 preproprotein
NCBI Official Synonym Full Names
growth differentiation factor 2
NCBI Official Symbol
GDF2
NCBI Official Synonym Symbols
BMP9; HHT5; BMP-9
NCBI Protein Information
growth/differentiation factor 2
UniProt Protein Name
Growth/differentiation factor 2
Protein Family
UniProt Gene Name
GDF2
UniProt Synonym Gene Names
BMP9; GDF-2; BMP-9
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. [provided by RefSeq, Jul 2016]
Uniprot Description
Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.
Research Articles on GDF2
Similar Products
Product Notes
The GDF2 gdf2 (Catalog #AAA553714) is an Active Protein produced from CHO and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GDF2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Recombinant GDF2 migrates as an approximately 12 kDa protein due to glycosylation under reducing conditions in SDS-PAGE. Researchers should empirically determine the suitability of the GDF2 gdf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SAGAGSHCQK TSLRVNFEDI GWDSWIIAPK E YEAYECKGGC FFPLADDVTP TK HAIVQTLVHL KFPTKVGKAC CVPTKLSPIS VLYKDDMGVP TLKYHYEGMS VAECGCR . It is sometimes possible for the material contained within the vial of "GDF2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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