IL7 active protein

IL7

Cat. Num. AAA553674

• Gene Names: IL7; IL-7
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: IL7; IL7 active protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: MNWIDVRYDL EKIESLIQSI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VAESGCKECE ELEEKTFTEF LQSFIRIVQM FINTS
• Sequence Length: 115

• Applicable Applications for IL7 active protein: SDS-PAGE
• Application Notes: Recombinant IL7 migrates as an approximately 13 kDa protein under reducing conditions in SDS-PAGE.
• Species: Rat
• Source Note: Optimized DNA sequence encoding Rat IL7 mature chain was expressed in ECOLI. Recombinant Rat Interleukin-7 encodes amino acid residues 26-154.
• Biological Activity: Determined by proliferation of murine CTLL-2 cells is < 5.0 ng/ml, corresponding to a specific activity of > 2 x 10^5 units/mg.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 315467871
• NCBI GeneID: 3574
• NCBI Accession #: NP_001186817.1
• NCBI GenBank Nucleotide #: NM_001199888.1
• UniProt Accession #: P13232; Q5FBY5; Q5FBY9; A0N0L3
• Molecular Weight: 15,203 Da
• NCBI Official Full Name: interleukin-7 isoform 4
• NCBI Official Synonym Full Names: interleukin 7
• NCBI Official Symbol: IL7
• NCBI Official Synonym Symbols: IL-7
• NCBI Protein Information: interleukin-7
• UniProt Protein Name: Interleukin-7
• Protein Family: Interleukin
• UniProt Gene Name: IL7
• UniProt Synonym Gene Names: IL-7

NCBI Description

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

Uniprot Description

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.