WISP1 active protein

WISP1

Cat. Num. AAA553638

• Gene Names: CCN4; WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: WISP1; WISP1 active protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN
• Sequence Length: 367

• Applicable Applications for WISP1 active protein: SDS-PAGE
• Application Notes: Recombinant WISP1 migrates as an approximately 38 kDa protein under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human WISP1 mature chain including a C-terminal 6xHis tag was expressed in ECOLI. Recombinant Human WNT1-inducible-signaling pathway protein 1 encodes amino acid residues 23-367.
• Biological Activity: Determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 4507921
• NCBI GeneID: 8840
• NCBI Accession #: NP_003873.1
• NCBI GenBank Nucleotide #: NM_003882.4
• UniProt Accession #: O95388; Q5JBS6; Q5JBS7; Q5JBS8; Q9HCS3; A8KAG6; E7EMM5
• Molecular Weight: 30,697 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 1 isoform 1
• NCBI Official Synonym Full Names: cellular communication network factor 4
• NCBI Official Symbol: CCN4
• NCBI Official Synonym Symbols: WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 1
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 1
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP1
• UniProt Synonym Gene Names: CCN4; WISP-1

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.