TNFRSF11B active protein

TNFRSF11B

Cat. Num. AAA553625

• Gene Names: TNFRSF11B; OPG; TR1; OCIF; PDB5
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: TNFRSF11B; TNFRSF11B active protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK
• Sequence Length: 401

• Applicable Applications for TNFRSF11B active protein: SDS-PAGE
• Application Notes: Recombinant TNFRSF11B migrates as an approximately 19 kDa protein under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human TNFRSF11B mature chain was expressed in ECOLI. Recombinant Human Tumor necrosis factor receptor superfamily member 11B encodes amino acid residues 22-401.
• Biological Activity: Determined by its ability to inhibit TRAIL-induced apoptosis of LN-18 glioblastoma cells
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 148743793
• NCBI GeneID: 4982
• NCBI Accession #: NP_002537.3
• NCBI GenBank Nucleotide #: NM_002546.4
• UniProt Accession #: O00300; O60236; Q53FX6; Q9UHP4; B2R9A8
• Molecular Weight: 46,026 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 11B
• NCBI Official Synonym Full Names: TNF receptor superfamily member 11b
• NCBI Official Symbol: TNFRSF11B
• NCBI Official Synonym Symbols: OPG; TR1; OCIF; PDB5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11B
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11B
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF11B
• UniProt Synonym Gene Names: OCIF; OPG

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.