Interleukin-25 Recombinant Protein | IL-25 recombinant protein
Mouse Interleukin-25 Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
Interleukin-25; Mouse Interleukin-25 Recombinant; il17e; SF20; il25; interleukin-25; il-25; IL-25 recombinant protein
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant mouse Interleukin-25 was lyophilized from 0.2um filtered concentrated (1.0mg/ml) solution in PBS, pH 7.0.
Sequence
MAAPSGGFWT AVVLAAAALK LAAAVSEPTT VPFDVRPGGV VHSFSQDVGP GNKFTCTFTY ASQGGTNEQW QMSLGTSEDS QHFTCTIWRP QGKSYLYFTQ FKAELRGAEI EYAMAYSKAA FERESDVPLK SEEFEVTKTA VSHRPGAFKA ELSKLVIVAK AARSEL
Sequence Length
166
Domain
4 Helix Cytokine
Host Note
Optimized DNA sequence encoding Mouse Interleukin-25 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native Mouse IL-25 has a calculated molecular mass of approximately 16 kDa.
Recombinant IL-25 is a disulfide-linked monomeric protein consisting of 143 amino acid residue subunits, and migrates as an approximately 16 kDa protein under reducing conditions in SDS-PAGE.
Recombinant IL-25 is a disulfide-linked monomeric protein consisting of 143 amino acid residue subunits, and migrates as an approximately 16 kDa protein under reducing conditions in SDS-PAGE.
Preparation and Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for IL-25 recombinant protein
IL-25 (also known as IL- 17E), a member of the IL-17 family, has been implicated in Th2 cell-mediated immunity. Recently, IL-25 has been identified as one of the initiators of the Th2 response (10, 11) and has also been shown to be expressed by mast cells. The transgenic overexpression of IL-25 by lung epithelial cells results in mucus hyperproduction and airway infiltration of macrophages and eosinophils; conversely, IL-25 blockade reduced airway inflammation and Th2 cytokine production in an allergen-induced asthma model.
Product Categories/Family for IL-25 recombinant protein
References
• t-helper cell type 2 (th2) memory t cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma .PNAS, Jan 2011; 10.1073/pnas.1014241108.
• critical role of IL-25 in nematode infection-induced alterations in intestinal function .J. Immunol., Dec 2010; 185: 6921 - 6929.
• nk cell deficiency predisposes to viral-induced th2-type allergic inflammation via epithelial-derived IL-25 .J. Immunol., Oct 2010; 185: 4681 - 4690.
• overexpression of smad2 drives house dust mite–mediated airway remodeling and airway hyperresponsiveness via activin and IL-25 .Am. J. Respir. Crit. Care Med., Jul 2010; 182: 143 - 154.
• pulmonary il-17e (IL-25) production and il-17rb+ myeloid cell-derived th2 cytokine production are dependent upon stem cell factor-induced responses during chronic allergic pulmonary disease .J. Immunol., Nov 2009; 183: 5705 - 5715.
• critical role of IL-25 in nematode infection-induced alterations in intestinal function .J. Immunol., Dec 2010; 185: 6921 - 6929.
• nk cell deficiency predisposes to viral-induced th2-type allergic inflammation via epithelial-derived IL-25 .J. Immunol., Oct 2010; 185: 4681 - 4690.
• overexpression of smad2 drives house dust mite–mediated airway remodeling and airway hyperresponsiveness via activin and IL-25 .Am. J. Respir. Crit. Care Med., Jul 2010; 182: 143 - 154.
• pulmonary il-17e (IL-25) production and il-17rb+ myeloid cell-derived th2 cytokine production are dependent upon stem cell factor-induced responses during chronic allergic pulmonary disease .J. Immunol., Nov 2009; 183: 5705 - 5715.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,982 Da
NCBI Official Full Name
UPF0556 protein C19orf10 homolog
UniProt Protein Name
UPF0556 protein C19orf10 homolog
Protein Family
UniProt Gene Name
D17Wsu104e
UniProt Synonym Gene Names
Il25; IL-25
UniProt Entry Name
CS010_MOUSE
NCBI Description
The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was identified in error as interleukin 25. This activity has not been reproducible, however, and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]
Uniprot Description
MYDGF: was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis
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Product Notes
The IL-25 d17wsu104e (Catalog #AAA553229) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MAAPSGGFWT AVVLAAAALK LAAAVSEPTT VPFDVRPGGV VHSFSQDVGP GNKFTCTFTY ASQGGTNEQW QMSLGTSEDS QHFTCTIWRP QGKSYLYFTQ FKAELRGAEI EYAMAYSKAA FERESDVPLK SEEFEVTKTA VSHRPGAFKA ELSKLVIVAK AARSEL. It is sometimes possible for the material contained within the vial of "Interleukin-25, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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