Vascular endothelial growth factor 165AA Recombinant Protein | VEGF recombinant protein

Human Vascular endothelial growth factor 165AA Recombinant

Cat. Num. AAA553223

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Vascular endothelial growth factor 165AA; Human Vascular endothelial growth factor 165AA Recombinant; vascular endothelial; vegf; VEGF recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant VEGF was lyophilized from 0.2 um filtered 20mM PB solution, pH 7.0.
• Sequence: MNFLLSWVHW SLALLLYLHH AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE NPCGPCSERR KHLFVQDPQT CKCSCKNTDS RCKARQLELN ERTCRCDKPR R
• Sequence Length: 412

• Domain: PDGF
• Host Note: Optimized DNA sequence encoding Human VEGF mature chain was expressed in Escherichia Coli
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native human VEGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant Vascular endothelial growth factor is a disulfide-linked homodimer protein consisting of 165 amino acid residue subunits, and migrates as an approximately 38 kDa protein under non-reducing and as 19kDa under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for VEGF recombinant protein

VEGF is a homodimeric heavily glycosylated protein.The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189, 206 amino acids, arising by alternative splicing of the mRNA.The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. The 165 amino acid form of the factor is the most common form in most tissues. Kaposi sarcomas express VEGF121 and VEGF165.

Product Categories/Family for VEGF recombinant protein

Cytokines

References

• vascular endothelial growth factor regulates germ cell survival during establishment of spermatogenesis in the bovine testis .Reproduction, Oct 2009; 138: 667 - 677.
• vascular endothelial growth factor expression and neovascularization in non—small cell lung carcinoma .International Journal of Surgical Pathology, Oct 2009; 17: 390 - 395.
• significantly higher levels of vascular endothelial growth factor (vegf) and shorter survival times for patients with primary operable triple-negative breast cancer .Ann. Onc., Oct 2009; 20: 1639 - 1646.
• analysis of in situ and ex vivo vascular endothelial growth factor receptor expression during experimental aortic aneurysm progression .Arterioscler Thromb Vasc Biol, Oct 2009; 29: 1452 - 1457.
• copper-induced regression of cardiomyocyte hypertrophy is associated with enhanced vascular endothelial growth factor receptor-1 signalling pathway .Cardiovasc Res, Oct 2009; 84: 54 - 63.