Epidermal Growth Factor Recombinant Protein | EGF recombinant protein

Human Epidermal Growth Factor Recombinant

Cat. Num. AAA553139

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: Epidermal Growth Factor; Human Epidermal Growth Factor Recombinant; egf; epidermal growth factor; EGF recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Epidermal Growth Factor was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.2.
• Sequence: REDDHHYSVR NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELRHAGHGQQ QKVIVVAVCV VVLVMLLLLS LWGAHYYRTQ KLLSKNPKNP YEESSRDVRS RRPADTEDGM SSCPQPWFVV IKEHQDLKNG GQPVAGEDGQ AADGSMQPTS WRQEPQLCGM GTEQGCWIPV SSDKGSCPQV MERSFHMPSY GTQTLEGGVE KPHSLLSANP LWQQRALDPP HQMELTQ
• Sequence Length: 1165

• Domain: EGF
• Host Note: Optimized DNA sequence encoding Human EGF mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human EGF, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 6 kDa.; ; ; Recombinant Epidermal Growth Factor is a disulfide-linked monomer protein consisting of 54 amino acid residue subunits, migrates as an approximately 6 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for EGF recombinant protein

Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF receptors are expressed in almost all types of tissues. Parietal endoderm, mature skeletal muscles, and hematopoietic tissues do not express the receptor.

Product Categories/Family for EGF recombinant protein

Cytokines

References

• thrombin induces EGF receptor expression and cell proliferation via a pkc () /c-src-dependent pathway in vascular smooth muscle cells .Arterioscler Thromb Vasc Biol, Oct 2009; 29: 1594 - 1601.
• dependence of EGF-induced increases in corneal epithelial proliferation and migration on gsk-3 inactivation .Invest. Ophthalmol. Vis. Sci., Oct 2009; 50: 4828 - 4835.
• internalization and intracellular sorting of the EGF receptor: a model for understanding the mechanisms of receptor trafficking .J. Cell Sci., Oct 2009; 122: 3433 - 3439.
• angiotensin ii maintains cerebral vascular relaxation via EGF receptor transactivation and erk1/2 .Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1296 - H1303.
• g protein-coupled receptor 30 expression is up-regulated by EGF and tgf in estrogen receptor -positive cancer cells .Mol. Endocrinol., Sep 2009; 10.1210/me.2009-0120.