HCC-2 (CCL15) Recombinant Protein | HCC-2 recombinant protein

Human HCC-2 (CCL15) Recombinant

Cat. Num. AAA553121

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: HCC-2 (CCL15); Human HCC-2 (CCL15) Recombinant; ccl-15 ; CCL15; hcc-2; hcc2; mip-5; mip5; HCC-2 recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant HCC-2 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
• Sequence: MKVSVAALSC LMLVAVLGSQ AQFINDAETE LMMSKLPLEN PVVLNSFHFA ADCCTSYISQ SIPCSLMKSY FETSSECSKP GVIFLTKKGR QVCAKPSGPG VQDCMKKLKP YSI
• Sequence Length: 113

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human HCC-2 (CCL15) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human HCC-2, generated by the proteolytic removal of the signal peptide; and propeptide and has a calculated molecular mass of approximately 10 kDa.; ; Recombinant HCC-2 is a disulfide-linked monomeric protein consisting of 92 amino acid residue subunits and migrates as an approximately 10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for HCC-2 recombinant protein

CCL15 is a human chemokine which acts predominantly on chemokine receptor CCR1 and to a lesser degree on CCR3. It is a potent chemoattractant for monocytes and lymphocytes in vitro and, after i.p. application, CCL15 induces recruitment neutrophils, monocytes, and lymphocytes into mouse peritoneum. CCL15 is constitutively expressed in the gut and in the liver and was identified to circulate in human plasma. The N terminus of CCL15 was found to be important to exert its full biological activity. HCC-2 shares significant sequence homology with CKb8 and the murine chemokines C10, CCF18/MRP-2, and macrophage inflammatory protein 1g, which all contain six instead of four conserved cysteines.

Product Categories/Family for HCC-2 recombinant protein

Cytokines

References

• hemofiltrate cc chemokines with unique biochemical properties: hcc-1/ccl14a and HCC-2/ccl15 .J. Leukoc. Biol., Sep 2001; 70: 357.