IL2RA (CD25) Active Protein | CD25 active protein

Human IL2RA Recombinant (CD25)

Cat. Num. AAA553092

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: IL2RA (CD25); Human IL2RA Recombinant (CD25); Human CD25 Recombinant (IL2Ra) ; CD25; IDDM10; IL2R; TCGFR; il-2R; CD25 active protein

• Host: HEk293 Cells
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CD25 is supplied as a 0.2 um filtered PBS solution, pH7.2.
• Sequence Length: 272

• Domain: Extracellular
• Host Note: Optimized DNA sequence encoding extracellular domain of human CD25 including a C-terminal polyHis tag was expressed in HEK293 cells
• Biological Activity: The activity of recombinant IL-2Ra (CD25) was determined by its ability to bind Human IL-2 with an affinity constant of 30nM.
• Presentation: Recombinant CD25 is supplied as a 0.2um filtered PBS solution pH 7.4.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to prepare a stock solution concentration not less than 0.1mg/ml.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Molecular Weight Note: Recombinant CD25 is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately 44 kDa protein on SDS-PAGE.
• Preparation and Storage: Recombinant CD25, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CD25 active protein

This cell surface antigen is known also as TAC antigen. It is expressed on T-cells and B-cells following cell activation by antigens. An old designation for the murine counterpart is Ly43. The rat antigen is OX39. CD25 is identical with IL2RA, which is the approved gene symbol. It is called also T- cell growth factor receptor (TCGFR)

Product Categories/Family for CD25 active protein

Receptors CD

References

• defects in il-2r signaling contribute to diminished maintenance of foxp3 expression in cd4+CD25+ regulatory t-cells of type 1 diabetic subjects .Diabetes, Feb 2010; 59: 407 - 415.
• il-2/anti-il-2 antibody complexes show strong biological activity by avoiding interaction with il-2 receptor subunit CD25 .PNAS, Jan 2010; 10.1073/pnas.0909384107.
• functional characterization of alloreactive t cells identifies CD25 and cd71 as optimal targets for a clinically applicable allodepletion strategy .Blood, Jan 2010; 115: 396 - 407.
• cd4+ t cell regulation of CD25 expression controls development of short-lived effector cd8+ t cells in primary and secondary responses .PNAS, Jan 2010; 107: 193 - 198.
• cd4+CD25+ regulatory t cells resist a novel form of cd28- and fas-dependent p53-induced t cell apoptosis .J. Immunol., Jan 2010; 184: 94 - 104.