Interleukin-15 Recombinant Protein | IL-15 recombinant protein

Human Interleukin-15 Recombinant

Cat. Num. AAA553005

• Purity: >96%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-15; Human Interleukin-15 Recombinant; il-15; il15; IL-15 recombinant protein

• Host: E Coli
• Purity/Purification: >96%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant IL-15 was lyophilized from a 0.2 um filtered PBS solution pH 7.5.
• Sequence: MRISKPHLRS ISIQCYLCLL LNSHFLTEAG IHVFILGCFS AGLPKTEANW VNVISDLKKI EDLIQSMHID ATLYTESDVH PSCKVTAMKC FLLELQVISL ESGDASIHDT VENLIILANN SLSSNGNVTE SGCKECEELE EKNIKEFLQS FVHIVQMFIN TS
• Sequence Length: 162

• Domain: Interleukin-15
• Host Note: Optimized DNA sequence encoding Human Interleukin-15 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Interleukin-15 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant Interleukin-15 is a disulfide-linked homodimeric protein consisting of two 115 amino acid residue subunits, and migrates as an approximately 13kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-15 recombinant protein

L15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL1- beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system. IL15 stimulates proliferation of the established T-cell line CTLL-2, CD8 (+) memory T- cells require IL15 for proliferation.IL15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. L15 induces proliferation of mast cells in the absence of functional IL2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL15 receptors. The IL15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3 and STAT3 and STAT5 that are activated in T- cells. The alpha subunit of the IL15 receptor (IL15RA) is structurally related to the alpha subunit of the IL2 receptor and confers high affinity binding of IL15 to its receptor. IL15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction.

Product Categories/Family for IL-15 recombinant protein

Cytokines

References

• dendritic cells support the in vivo development and maintenance of nk cells via IL-15 trans-presentation .J. Immunol., Sep 2009; 10.4049/jimmunol.0900719.
• safety and immunologic effects of IL-15 administration in nonhuman primates .Blood, Sep 2009; 114: 2417 - 2426.
• cutting edge: tgf-β1 and IL-15 induce foxp3+ regulatory t cells in the presence of antigen stimulation .J. Immunol., Sep 2009; 183: 3574 - 3577.
• antibody-mediated blockade of IL-15 reverses the autoimmune intestinal damage in transgenic mice that overexpress IL-15 in enterocytes .PNAS, Sep 2009; 106: 15849 - 15854.
• high antitumor activity of rli, an interleukin-15 (IL-15) –IL-15 receptor fusion protein, in metastatic melanoma and colorectal cancer .Mol. Cancer Ther., Sep 2009; 8: 2736 - 2745.