Immunohistochemistry
Rabbit alpha Synuclein Polyclonal Antibody | anti-SNCA antibody
Rabbit anti alpha Synuclein polyclonal
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of FFPE rat brain sections showing expression of alpha Synuclein.
It was used as primary antibody, dilution 1:500, DAB staining.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Immunohistochemistry
Immunohistochemistry
• serine 129 phosphorylation reduces the ability of -synuclein to regulate Tyrosine Hydroxylase and protein phosphatase 2a in vitro and in vivo .J. Biol. Chem., Jun 2010; 285: 17648 - 17661.
• Tyrosine Hydroxylase is expressed during early heart development and is required for cardiac chamber formation .Cardiovasc Res, Jun 2010; 10.1093/cvr/cvq179.
• Tyrosine Hydroxylase deficiency: a treatable disorder of brain catecholamine biosynthesis .Brain, Jun 2010; 133: 1810 - 1822.
• electrophysiological and morphological characteristics and synaptic connectivity of Tyrosine Hydroxylase-expressing neurons in adult mouse striatum .J. Neurosci., May 2010; 30: 6999 - 7016.
NCBI and Uniprot Product Information
NCBI Description
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: Golgi apparatus; nuclear outer membrane; mitochondrion; rough endoplasmic reticulum; lysosome; fibril; extracellular region; terminal button; inclusion body; cell cortex; cytosol; mitochondrial respiratory chain complex I; actin cytoskeleton; synaptic vesicle; platelet alpha granule membrane; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; nucleus; cell junction
Molecular Function: protein domain specific binding; identical protein binding; histone binding; zinc ion binding; kinesin binding; microtubule binding; ferrous iron binding; caspase inhibitor activity; magnesium ion binding; beta-tubulin binding; phosphoprotein binding; protein N-terminus binding; Hsp70 protein binding; oxidoreductase activity; calcium ion binding; dynein binding; protein binding; copper ion binding; phospholipase binding; phospholipid binding; fatty acid binding; tau protein binding; alpha-tubulin binding
Biological Process: regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; regulation of acyl-CoA biosynthetic process; positive regulation of apoptosis; adult locomotory behavior; negative regulation of norepinephrine uptake; mitochondrial membrane organization and biogenesis; microglial cell activation; response to lipopolysaccharide; positive regulation of endocytosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; negative regulation of monooxygenase activity; dopamine biosynthetic process; fatty acid metabolic process; regulation of dopamine secretion; positive regulation of neurotransmitter secretion; negative regulation of histone acetylation; negative regulation of dopamine uptake; calcium ion homeostasis; response to magnesium ion; negative regulation of exocytosis; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; synapse organization and biogenesis; fibril organization and biogenesis; dopamine uptake; response to iron(II) ion; negative regulation of neuron apoptosis; positive regulation of receptor recycling; aging; caspase activation; response to drug; neutral lipid metabolic process; protein destabilization; regulation of macrophage activation; negative regulation of microtubule polymerization; regulation of glutamate secretion; positive regulation of peptidyl-serine phosphorylation; negative regulation of dopamine metabolic process; organelle ATP synthesis coupled electron transport; regulation of locomotion; synaptic vesicle endocytosis; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential; negative regulation of transporter activity; negative regulation of apoptosis
Disease: Parkinson Disease 4, Autosomal Dominant; Parkinson Disease 1, Autosomal Dominant; Dementia, Lewy Body
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Product Notes
The SNCA snca (Catalog #AAA551154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti alpha Synuclein polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Synuclein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA). ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat brain sections showing expression of alpha Synuclein. It was used as primary antibody, dilution 1:500, DAB staining. Researchers should empirically determine the suitability of the SNCA snca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Synuclein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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