Rabbit ErbB 2 Polyclonal Antibody | anti-ErbB 2 antibody

ErbB 2 Antibody FITC-Conjugated

Cat. Num. AAA541545

• Gene Names: ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• Reactivity: Human, Mouse; Predicted: Rat
• Applications: ELISA, Immunoprecipitation, Western Blot
• Synonyms: ErbB 2; Polyclonal Antibody; ErbB 2 Antibody FITC-Conjugated; Verb b2 erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog; CD340; CerbB2; cneu; Erb B2; erbb2; Erythroblastosis Virus; HER 2; Herstatin; Metastatic lymph node gene 19 protein; MLN 19; NEU; Neuro Glioblastoma Derived Oncogene Homolog; NGL; p185 ErbB2; Proto-oncogene Neu; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Tyrosine kinase-type cell surface receptor HER2; V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody; anti-ErbB 2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Rat
• Clonality: Polyclonal
• Form/Format: FITC-Conjugated
• Concentration: 0.65-1.25 ug/ul in antibody stabilization buffer (varies by lot)
• Sequence Length: 1255

• Applicable Applications for anti-ErbB 2 antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 1:10,000; Immunoprecipitation: 1:200; Western Blot: 1:500
• Immunogen: Synthetic peptide taken within amino acid region 1125-1175 receptor tyrosine-protein kinase erbB-2 protein.
• Immunoglobulin: IgG
• Expression: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
• Determinant: N-epitope
• Molecular Function: Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase
• Structure: Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).
• Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm;perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.
• Preparation and Storage: -20 degree C for long term storage

Related Product Information for anti-ErbB 2 antibody

FITC-Conjugated Receptor tyrosine-protein kinase erB-2 isoform a Antibody N-epitope
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

NCBI and Uniprot Product Information

• NCBI GI #: 54792096
• NCBI GeneID: 2064
• NCBI Accession #: NP_004439.2
• NCBI GenBank Nucleotide #: NM_004448.3
• UniProt Accession #: P04626; Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3; X5D2V5
• Molecular Weight: 97,382 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2 isoform a
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 2
• NCBI Official Symbol: ERBB2
• NCBI Official Synonym Symbols: NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• UniProt Gene Name: ERBB2
• UniProt Synonym Gene Names: HER2; MLN19; NEU; NGL; MLN 19
• UniProt Entry Name: ERBB2_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Oncoprotein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; TK group; EGFR family

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasm; cytoplasmic vesicle; endosome membrane; integral to membrane; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex

Molecular Function: ATP binding; ErbB-3 class receptor binding; growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein C-terminus binding; protein dimerization activity; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell proliferation; cell surface receptor linked signal transduction; enzyme linked receptor protein signaling pathway; heart development; MAPKKK cascade; motor axon guidance; myelination; negative regulation of immature T cell proliferation in the thymus; neuromuscular junction development; oligodendrocyte differentiation; peptidyl-tyrosine phosphorylation; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of angiogenesis; regulation of microtubule-based process; regulation of phosphoinositide 3-kinase cascade; signal transduction; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing

Disease: Gastric Cancer; Glioma Susceptibility 1; Lung Cancer