Rabbit SIRT5 Polyclonal Antibody | anti-SIRT5 antibody

SIRT5 Antibody

Cat. Num. AAA540427

• Reactivity: Chicken, Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot
• Synonyms: SIRT5; Polyclonal Antibody; SIRT5 Antibody; NAD dependent deacetylase sirtuin 5; Regulatory protein SIR2 homolog 5; Silent mating type information regulation 2 Sir2 like 5; SIR2-like protein 5; SIR2L5; Sirtuin type 5 antibody; anti-SIRT5 antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 0.66-0.68 ug/ul in antibody stabilization buffer (varies by lot)
• Sequence Length: 310

• Applicable Applications for anti-SIRT5 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Dot Blot: 1:10,000; ELISA: 1:10,000; Western Blot: 1:500
• Immunogen: Synthetic peptide taken within amino acid region 280-320 on rat Sirtuin 5 protein.
• Expression: Widely expressed.
• Molecular Function: Hydrolase; Metal-binding; NAD; Zinc
• Structure: Interacts with CPS1 (By similarity). Monomer. Homodimer. Forms homodimers upon suramin binding.
• Subcellular Location: Isoform 1: Cytoplasm, Mitochondrion Isoform 2: Mitochondrion
• Preparation and Storage: -20 degree C for long term storage

Related Product Information for anti-SIRT5 antibody

Affinity Purified Sirtuin 5 (silent mating type information regulation 2 homolog) 5 Antibody
NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.

Product Categories/Family for anti-SIRT5 antibody

Primary Antibodies; Epigenetics; Chromatin Modifying Enzymes; Acetylation; SIRT5 Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 149045113
• NCBI GeneID: 306840
• Molecular Weight: 34,098 Da
• NCBI Official Full Name: sirtuin 5 (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• NCBI Official Synonym Full Names: sirtuin 5
• NCBI Official Symbol: Sirt5
• NCBI Protein Information: NAD-dependent protein deacylase sirtuin-5, mitochondrial
• UniProt Protein Name: NAD-dependent protein deacylase sirtuin-5, mitochondrial
• Protein Family: NAD-dependent protein deacylase sirtuin
• UniProt Gene Name: Sirt5
• UniProt Entry Name: SIR5_RAT

Uniprot Description

SIRT5: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD- dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. Belongs to the sirtuin family. Class III subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 3.5.1.-; Mitochondrial

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; nucleus

Molecular Function: zinc ion binding

Biological Process: protein amino acid deacetylation; response to nutrient levels