Rabbit SIRT5 Polyclonal Antibody | anti-SIRT5 antibody
SIRT5 Antibody
Reactivity
Chicken, Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot
Synonyms
SIRT5; Polyclonal Antibody; SIRT5 Antibody; NAD dependent deacetylase sirtuin 5; Regulatory protein SIR2 homolog 5; Silent mating type information regulation 2 Sir2 like 5; SIR2-like protein 5; SIR2L5; Sirtuin type 5 antibody; anti-SIRT5 antibody
Host
Rabbit
Reactivity
Chicken, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
0.66-0.68 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
310
Applicable Applications for anti-SIRT5 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Western Blot: 1:500
ELISA: 1:10,000
Western Blot: 1:500
Immunogen
Synthetic peptide taken within amino acid region 280-320 on rat Sirtuin 5 protein.
Expression
Widely expressed.
Molecular Function
Hydrolase; Metal-binding; NAD; Zinc
Structure
Interacts with CPS1 (By similarity). Monomer. Homodimer. Forms homodimers upon suramin binding.
Subcellular Location
Isoform 1: Cytoplasm, Mitochondrion Isoform 2: Mitochondrion
Preparation and Storage
-20 degree C for long term storage
Related Product Information for anti-SIRT5 antibody
Affinity Purified Sirtuin 5 (silent mating type information regulation 2 homolog) 5 Antibody
NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.
NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.
Product Categories/Family for anti-SIRT5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,098 Da
NCBI Official Full Name
sirtuin 5 (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
NCBI Official Synonym Full Names
sirtuin 5
NCBI Official Symbol
Sirt5
NCBI Protein Information
NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Protein Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
Protein Family
UniProt Gene Name
Sirt5
UniProt Entry Name
SIR5_RAT
Uniprot Description
SIRT5: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD- dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. Belongs to the sirtuin family. Class III subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Deacetylase; EC 3.5.1.-; Mitochondrial
Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; nucleus
Molecular Function: zinc ion binding
Biological Process: protein amino acid deacetylation; response to nutrient levels
Research Articles on SIRT5
Similar Products
Product Notes
The SIRT5 sirt5 (Catalog #AAA540427) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT5 Antibody reacts with Chicken, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Western Blot: 1:500. Researchers should empirically determine the suitability of the SIRT5 sirt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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