Testing Data (TD)
CXCR3 blocking peptide
CXCR3 Blocking Peptide
WB: 1:500
Testing Data (TD)
Testing Data (TD)
Chemokines (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hematopoietic cells. Chemokine receptors (CXCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors articipate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases.
Activation of naive T cells by specific antigen and cytokines, up-regulate cell adhesion molecules (CAM) as well as chemokine receptors on their surface, which directs them to migrate towards the inflamed tissues. The CXCR3 receptor protein is approximately 40-45 kDa protein which has 7 transmembrane domains, characteristic of G-protein coupled receptors. The protein has putative N-glycosylation sites near the extracellular N-terminal end of the protein. The protein has a large 3rd intracellular loop which nteracts with G-proteins. The short carboxyl terminal is intracellular and has putative post-translational sites.
The Anti-CXCR3h-selective antibodies were generated against unique peptide sequence from the CXCR3 receptors gene that is expressed only in a CXCR3 receptor subtype of the human protein. The polyclonal antibodies were affinity purified on an immobilized antigen based affinity chromatography. Antigenic blocking peptides (MBS5401341) and western blot positive control (MBS542665) in ready to use SDS-sample buffers are available. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]
Uniprot Description
CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: Xq13
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane
Molecular Function: C-X-C chemokine binding; chemokine binding; chemokine receptor activity; receptor activity; C-X-C chemokine receptor activity
Biological Process: apoptosis; positive regulation of chemotaxis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of leukocyte migration; negative regulation of endothelial cell proliferation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of release of sequestered calcium ion into cytosol; angiogenesis; cell motility; inflammatory response; cell adhesion
Research Articles on CXCR3
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Product Notes
The CXCR3 cxcr3 (Catalog #AAA5401341) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CXCR3 can be used in a range of immunoassay formats including, but not limited to, Immunodepletion, Immunocompetition, Immunohistochemistry, Western Blot. IHC: 1:250 WB: 1:500. Researchers should empirically determine the suitability of the CXCR3 cxcr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCR3, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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