CARD15 positive control

CARD15 Positive Control

Cat. Num. AAA5400737

• Gene Names: Nod2; CD; ACUG; BLAU; IBD1; Nlrc2; Card15; F830032C23Rik
• Applications: Western Blot
• Synonyms: CARD15; CARD15 Positive Control; Western Blot Positive Control Nucleotide-binding oligomerization domain-containing protein 2 Antibody; ACUG antibody; Arthrocutaneouveal granulomatosis antibody; BLAU antibody; CARD15 antibody; Caspase recruitment domain protein 15 antibody; CD antibody; CLR16.3 antibody; IBD1 antibody; Inflammatory bowel disease protein 1 antibody; LRR containing protein antibody; NLR family; CARD domain containing 2 antibody; NLRC2 antibody; NOD like receptor C2 antibody; NOD2 antibody; NOD2B antibody; Nucleotide binding oligomerization domain; leucine rich repeat and CARD domain containing 2 antibody; PSORAS1 antibody; CARD15 positive control

• Sequence Length: 1045

• Applicable Applications for CARD15 positive control: Western Blot (WB)
• Application Notes: WB: 1:2,500-1:5,000
• Immunogen: Purified Protein
• Molecular Function: actin binding; ATP binding; CARD domain binding; endodeoxyribonuclease activity; producing 5'-phosphomonoesters; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; muramyl dipeptide binding; peptidoglycan binding; protein kinase binding
• Subcellular Location: Cytoplasm; Membrane; Basolateral cell membrane
• Preparation and Storage: Store at -20 degree C for long term storage.

Related Product Information for CARD15 positive control

Overview: Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response (PubMed:22607974). Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561).

Structure: The ATG16L1-binding motif mediates interaction with ATG16L1. Intramolecular interactions between the N-terminal moiety and the leucine-rich repeats (LRR) may be important for autoinhibition in the absence of activating signal. In the absence of LRRs, the protein becomes a constitutive activator of CASP1 cleavage and proIL1B processing.

NCBI and Uniprot Product Information

• NCBI GI #: 28386271
• NCBI GeneID: 257632
• UniProt Accession #: Q8K3Z0
• Molecular Weight: 115,301 Da
• NCBI Official Full Name: Nod2 protein, partial
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 2
• NCBI Official Symbol: Nod2
• NCBI Official Synonym Symbols: CD; ACUG; BLAU; IBD1; Nlrc2; Card15; F830032C23Rik
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 2
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 2
• UniProt Gene Name: Nod2
• UniProt Synonym Gene Names: Card15

Uniprot Description

Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response (PubMed:22607974). Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561). Component of an autophagy-mediated antibacterial pathway together with ATG16L1. Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon.