Rabbit VPRBP Polyclonal Antibody | anti-VPRBP antibody
VPRBP Antibody BIOTIN-Conjugated
Reactivity
Human Immunodeficiency Virus 1
Applications
ELISA, Western Blot
Synonyms
VPRBP; Polyclonal Antibody; VPRBP Antibody BIOTIN-Conjugated; DCAF1; DDB1 and CUL4 associated factor 1; HIV-1 Vpr-binding protein; KIAA0800; protein VPRBP; RIP; Vpr (HIV-1) binding protein; VPR BP; Vpr interacting protein; Vpr-binding protein antibody; anti-VPRBP antibody
Host
Rabbit
Reactivity
Human Immunodeficiency Virus 1
Clonality
Polyclonal
Form/Format
BIOTIN-Conjugated
Concentration
0.52 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
27
Applicable Applications for anti-VPRBP antibody
ELISA (EIA), IMM, Western Blot (WB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunogen
Synthetic peptide taken within amino acid region 1-27 on human VPR protein.
Molecular Function
Viral Life Cycle
Structure
Homooligomer, may form homodimer (PubMed:7798208). Interacts with p6-gag region of the Pr55 Gag precursor protein through a (Leu-X-X)4 motif near the C terminus of the P6gag protein (PubMed:7474102). Interacts with host UNG (PubMed:9151883). May interact with host RAD23A/HHR23A (PubMed:9371639). Interacts with host VPRBP/DCAF1, leading to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediate ubiquitination of host proteins such as TERT and ZGPAT and arrest the cell cycle in G2 phase (PubMed:17314515, PubMed:17630831, PubMed:17314515).
Subcellular Location
Virion; Host nucleus; Host Extracellular space
Preparation and Storage
-20 degree C for long term storage
Related Product Information for anti-VPRBP antibody
BIOTIN-Conjugated VPR Antibody
During virus entry, involved in the transport of the viral pre-integration (PIC) complex to the nucleus (PubMed:12417576). This function is crucial for viral infection of non-dividing macrophages (PubMed:12417576). May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions. During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest(PubMed:7666531, PubMed:7474100). Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the Vpr carried into the virion. Additionally, Vpr induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, Vpr may also induce cell death to bystander cells (PubMed:11000244).
During virus entry, involved in the transport of the viral pre-integration (PIC) complex to the nucleus (PubMed:12417576). This function is crucial for viral infection of non-dividing macrophages (PubMed:12417576). May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions. During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest(PubMed:7666531, PubMed:7474100). Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the Vpr carried into the virion. Additionally, Vpr induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, Vpr may also induce cell death to bystander cells (PubMed:11000244).
NCBI and Uniprot Product Information
Similar Products
Product Notes
The VPRBP vpr (Catalog #AAA5400226) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPRBP Antibody BIOTIN-Conjugated reacts with Human Immunodeficiency Virus 1 and may cross-react with other species as described in the data sheet. AAA Biotech's VPRBP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), IMM, Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the VPRBP vpr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPRBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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