Human Bordetella pertussis IgA ELISA Kit
Bordetella pertussis IgA ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
Bordetella pertussis IgA; Bordetella pertussis IgA ELISA Kit; Bordetella pertussis IgA elisa kit
Reactivity
Human
Applicable Applications for Bordetella pertussis IgA elisa kit
ELISA (EIA)
Application Notes
The Bordetella pertussis IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Bordetella antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Bordetella antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgA antibodies is directly proportional to the intensity of the color.
Samples
Serum
Assay Type
Quantitative Sandwich
Preparation and Storage
Store at 4 degree C.
Related Product Information for Bordetella pertussis IgA elisa kit
Principle of the Assay: The Anti-Spermatozoa Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) Ig-Classifying Test from MBS is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of antispermatozoa antibodies in human serum. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and controls are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilized on the plate. An enzyme conjugate containing antiserum directed against different regions of human immunoglobulins of different classes (IgA, IgG, IgM) and POD binds to the antigen-antibody-complex during the incubation. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3',5,5'-tetramethylbenzidine) yielding a color reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ?550 nm is recommended!!Background/Introduction: The Anti-Spermatozoa Antibody ELISA Ig-Classifying Test from MBS is a reliable and quantitative test for the determination of immunoglobulin class specific antibodies directed against human spermatozoa. This test is intended for the use with serum. Please note: the terms "anti-spermatozoa antibodies", "anti-sperm antibodies" and "sperm antibodies" are equivalent. In these instructions the rather unwieldy but correct term "anti-spermatozoa antibodies" is used.
Product Categories/Family for Bordetella pertussis IgA elisa kit
Similar Products
Product Notes
The Human Bordetella pertussis IgA (Catalog #AAA539735) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA539735 ELISA Kit recognizes Human Bordetella pertussis IgA. AAA Biotech's Bordetella pertussis IgA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). The Bordetella pertussis IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Bordetella antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Bordetella antigen takes place. After an one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgA antibodies is directly proportional to the intensity of the color. Researchers should empirically determine the suitability of the Bordetella pertussis IgA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bordetella pertussis IgA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
