CMV elisa kit
CMV ELISA Kit
Applications
ELISA
Synonyms
CMV; CMV ELISA Kit; Cytomegalovirus ELISA Kit; CMV elisa kit
Applicable Applications for CMV elisa kit
ELISA (EIA)
Application Notes
Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
Preparation and Storage
Store at 4 degree C.
Related Product Information for CMV elisa kit
Principle of the Assay: Differences in cell density are exploited to separate granulocytes and erythrocytes from peripheral blood mononuclear cells (PBMCs). The most common technique for separating PBMCs is to mix blood with a compound which aggregates the erythrocytes, thereby increasing their sedimentation rate. The active ingredients in the SepOBuffer are sodium diatrizoate and polysaccharide with osmolality 290 +15 mOsm/L and density of 1.077 +0.001 g/ml. The polysaccharide in SepOBuffer enhances erythrocyte aggregation increasing erythrocyte sedimentation during centrifugation. PBMCs with lower densities remain in the plasma SepOBuffer interface and can be collected from the upper part of the tube when the erythrocytes have settled. The PBMCs from whole blood can be reliably and rapidly isolated in pure form using SepOBuffer.
Product Categories/Family for CMV elisa kit
Similar Products
Product Notes
The CMV (Catalog #AAA539725) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CMV can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. Researchers should empirically determine the suitability of the CMV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CMV, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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