Goat anti Human IgE (epsilon chain) (HRP) Secondary Antibody | IgE secondary antibody
Goat anti Human IgE (epsilon chain) (HRP)
Reactivity
Reacts with heavy (Sigma) chains on Human IgE. Does not react iwth light chains on all Human immunoflobulins, or non-immunoglobulin Human serum proteins.
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified using solid phase Human IgE
Synonyms
Goat anti Human IgE (epsilon chain) (HRP); Goat anti Human Immunoglobulin E (epsilon chain) secondary (HRP); IgE secondary antibody
Host
Goat
Reactivity
Reacts with heavy (Sigma) chains on Human IgE. Does not react iwth light chains on all Human immunoflobulins, or non-immunoglobulin Human serum proteins.
Specificity
This antibody reacts with heavy chains on human IgE (epsilon chain)
Purity/Purification
Affinity purified using solid phase Human IgE
Form/Format
1.0mg/ml lyophilized from 10mM Sodium Phospahte, pH7.2 with 150mM Soidum Chloride, with 1% BSA and 0.1% Kathon CG.(use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.). Rehydrate with 1.1 ml og deionized water. Allow reconstituted
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for IgE secondary antibody
ELISA, Western Blot (WB), Immunohistochemistry (IHC), CL
Application Notes
IHC: 1:200-1:5000
ELISA/WB: 1:500-1:5000
ELISA/WB: 1:500-1:5000
Source Note
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Biological Significance
IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal non-atopic individual are only 0.05% of the Ig concentration, compared to 10 mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful immune reactions.
Cross-Reactivity
No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins.
Tag/Conjugate
HRP
Immunogen
Goat anti-Human IgE (epsilon chain) (HRP) was raised in goat using purified Human IgE as the immunogen.
Preparation and Storage
4 degree C. After reconsitution, dilute with an equal volume of glycerol and store at -20 degree C. Prepare working dilution prior to use and then discard.
Related Product Information for IgE secondary antibody
Goat anti Human IgE (epsilon chain) secondary antibody (HRP)
Product Categories/Family for IgE secondary antibody
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Product Notes
The IgE (Catalog #AAA539132) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Human IgE (epsilon chain) (HRP) reacts with Reacts with heavy (Sigma) chains on Human IgE. Does not react iwth light chains on all Human immunoflobulins, or non-immunoglobulin Human serum proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Goat anti Human IgE (epsilon chain) (HRP) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB), Immunohistochemistry (IHC), CL. IHC: 1:200-1:5000 ELISA/WB: 1:500-1:5000. Researchers should empirically determine the suitability of the IgE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat anti Human IgE (epsilon chain) (HRP), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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