HIV1-RT active protein
HIV1-RT protein
Applications
User optimized
Synonyms
HIV1-RT; HIV1-RT protein; HIV-1 RT; HIV 1-RT; HIV-1 RT protein; HIV1RT protein; HIV1 RT protein; HIV 1-RT protein; HIV-1 Reverse Transcriptase protein; HIV1-RT active protein
Host
Viral
Form/Format
Lyophilized from 25mM Tris, 150mM NaCl, pH 7.4. Reconstitute with sterile distilled water.
Applicable Applications for HIV1-RT active protein
User optimized
Protein Type
Recombinant
Biological Significance
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
Specific Activity
Approximately 100,000 TCA perceptible counts incorporated per 2ug of protein under standard assay conditions.
Expression System
E Coli
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for HIV1-RT active protein
Purified recombinant HIV1-RT protein
Product Categories/Family for HIV1-RT active protein
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Product Notes
The HIV1-RT (Catalog #AAA537337) is an Active Protein produced from Viral and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIV1-RT can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the HIV1-RT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV1-RT, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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