Western Blot
(Western Blot analysis using Carboxypeptidase Y antibodyBoth the antiserum and IgG fractions of anti-Carboxypeptidase Y are shown to detect under reducing conditions of SDS-PAGE the 61 KDa enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using Goat anti Rabbit IgG (H + L) (HRP) diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results. )
Rabbit Carboxypeptidase Y Polyclonal Antibody | anti-CPY antibody
Carboxypeptidase Y antibody
Reactivity
Saccharomyces cerevisiae carboxypeptidase Y
Applications
ELISA, Western Blot
Purity
Carboxypeptidase Y antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Synonyms
Carboxypeptidase Y; Polyclonal Antibody; Carboxypeptidase Y antibody; Polyclonal Carboxypeptidase Y; Anti-Carboxypeptidase Y; Carboxypeptidase YSCY; CPY1; Vacuolar carboxypeptidase Y; LBC1; PRC1; YMR297W; anti-CPY antibody
Host
Rabbit
Reactivity
Saccharomyces cerevisiae carboxypeptidase Y
Clonality
Polyclonal
Specificity
Saccharomyces cerevisiae carboxypeptidase Y
Purity/Purification
Carboxypeptidase Y antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form/Format
0.02M K3PO4, pH 7.2, with 0.15M NaCl and 0.01% NaN3.
Sequence Length
537
Applicable Applications for anti-CPY antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 10,000-1:42,000
WB: 1:5,000
WB: 1:5,000
Biological Significance
A carboxypeptidase is a protease enzyme that hydrolyzes (cleaves) the peptide bond of an amino acid residue at the carboxy-terminal (C-terminal) end. (Contrast with an aminopeptidase, which cleaves peptide bonds at the other end of the residue.) Humans, animals, and plants contain several types of carboxypeptidases which have diverse functions ranging from catabolism to protein maturation.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Carboxypeptidase Y antibody was raised in rabbit using Carboxypeptidase Y [Baker's Yeast] as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Western Blot
(Western Blot analysis using Carboxypeptidase Y antibodyBoth the antiserum and IgG fractions of anti-Carboxypeptidase Y are shown to detect under reducing conditions of SDS-PAGE the 61 KDa enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using Goat anti Rabbit IgG (H + L) (HRP) diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results. )
Western Blot
(Western Blot analysis using Carboxypeptidase Y antibodyBoth the antiserum and IgG fractions of anti-Carboxypeptidase Y are shown to detect under reducing conditions of SDS-PAGE the 61 KDa enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using Goat anti Rabbit IgG (H + L) (HRP) diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results. )
Related Product Information for anti-CPY antibody
Rabbit polyclonal Carboxypeptidase Y antibody
Product Categories/Family for anti-CPY antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
60,270 Da
NCBI Official Full Name
carboxypeptidase Y
UniProt Protein Name
Carboxypeptidase Y
Protein Family
UniProt Gene Name
CPY
UniProt Entry Name
O14414_PICAN
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Product Notes
The Carboxypeptidase Y cpy (Catalog #AAA535268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Carboxypeptidase Y antibody reacts with Saccharomyces cerevisiae carboxypeptidase Y and may cross-react with other species as described in the data sheet. AAA Biotech's Carboxypeptidase Y can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 10,000-1:42,000 WB: 1:5,000. Researchers should empirically determine the suitability of the Carboxypeptidase Y cpy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carboxypeptidase Y, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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