Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human leiomyoma stained with Actin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.)
Mouse Actin Monoclonal Antibody
Actin antibody (biotin)
Reactivity
Baboon, Chicken, Cow, Human, Mouse, Rabbit, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Actin antibody (biotin) was purified by Protein G chromatography.
Synonyms
Actin; Monoclonal Antibody; Actin antibody (biotin); Monoclonal Actin; Anti-Actin; b-actin; beta-actin; ACTB; beta Actin; anti-Actin antibody
Host
Mouse
Reactivity
Baboon, Chicken, Cow, Human, Mouse, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
1A4
Purity/Purification
Actin antibody (biotin) was purified by Protein G chromatography.
Form/Format
Antibody purified from ascites fluid by protein G chromotography and supplied in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% NaN3.
Concentration
40 ug/ml (varies by lot)
Applicable Applications for anti-Actin antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes
IHC-F: 1:800
IHC-P: 1:800
IHC-P: 1:800
Biological Significance
Actin is a globular, roughly 42- kDa protein found in eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells.
Cross-Reactivity
Baboon, chicken, cow, human, mouse, rabbit, rat
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
Tag/Conjugate
Biotin
Immunogen
Actin antibody (biotin) was raised in mouse using N-Terminal decapeptide of alpha smooth muscle isoform of actin, acetylated at the N-terminus, as the immunogen.
Preparation and Storage
Store at 4 degree C.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human leiomyoma stained with Actin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human leiomyoma stained with Actin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.)
Related Product Information for anti-Actin antibody
Mouse monoclonal Actin antibody (biotin)
Product Categories/Family for anti-Actin antibody
Similar Products
Product Notes
The Actin (Catalog #AAA531739) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin antibody (biotin) reacts with Baboon, Chicken, Cow, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Actin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin. IHC-F: 1:800 IHC-P: 1:800. Researchers should empirically determine the suitability of the Actin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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