Western Blot (WB)
(Western blot analysis of GRLF1 expression in HeLa (A); mouse brain (B) whole cell lysates.)
Rabbit GRLF1 Polyclonal Antibody | anti-GRLF1 antibody
GRLF1 Antibody
Gene Names
ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
Applications
Western Blot
Purity
Immunogen Affinity Chromatography
Synonyms
GRLF1; Polyclonal Antibody; GRLF1 Antibody; Purified Polyclonal GRLF1 Antibody; Polyclonal GRLF1 antibody; Anti-GRLF1 antibody; GRF1 antibody; GRLF1 antibody; KIAA1722 antibody; Rho GTPase-activating protein 35 antibody; Glucocorticoid receptor DNA-binding factor 1 antibody; Glucocorticoid receptor repression factor 1 antibody; GRF-1 antibody; Rho GAP p190A antibody; p190-A antibody; anti-GRLF1 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GRLF1 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
1499
Applicable Applications for anti-GRLF1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
GRLF1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GRLF1 as the immunogen
Cross-Reactivity
Mouse, Rat, Dog, Pig
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of GRLF1 expression in HeLa (A); mouse brain (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GRLF1 expression in HeLa (A); mouse brain (B) whole cell lysates.)
Related Product Information for anti-GRLF1 antibody
GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.
Product Categories/Family for anti-GRLF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
GRLF1 protein
NCBI Official Synonym Full Names
Rho GTPase activating protein 35
NCBI Official Symbol
ARHGAP35
NCBI Official Synonym Symbols
GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
NCBI Protein Information
rho GTPase-activating protein 35
NCBI Description
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Research Articles on GRLF1
Similar Products
Product Notes
The GRLF1 (Catalog #AAA5314442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GRLF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the GRLF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRLF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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