Testing Data
(HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse GCLM Monoclonal Antibody | anti-GCLM antibody
GCLM antibody
Applications
ELISA, Western Blot
Purity
Purified by protein-G affinity chromatography
Synonyms
GCLM; Monoclonal Antibody; GCLM antibody; Mouse monoclonal GCLM antibody; Monoclonal GCLM antibody; Anti-GCLM antibody; Glutamate-cysteine ligase antibody; GLCLR antibody; anti-GCLM antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT2D12
Purity/Purification
Purified by protein-G affinity chromatography
Form/Format
Liquid form in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-GCLM antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:3000
Immunogen
GCLM antibody was purified by recombinant human GCLM (1-274aa) purified from E Coli
Cross-Reactivity
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at-20 degree C.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Testing Data
(HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Testing Data
(HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-GCLM antibody
GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.
Product Categories/Family for anti-GCLM antibody
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Product Notes
The GCLM (Catalog #AAA5310006) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GCLM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:3000. Researchers should empirically determine the suitability of the GCLM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCLM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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