Mouse PBX1 Monoclonal Antibody | anti-PBX1 antibody

Mouse monoclonal PBX1 antibody

Cat. Num. AAA5306996

• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PBX1; Monoclonal Antibody; Mouse monoclonal PBX1 antibody; PBX1 antibody; anti-PBX1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1D9
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 430

• Applicable Applications for anti-PBX1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; WB: 1:2000
• Immunogen: PBX1 antibody was raised in mouse using a full length recombinant protein of human PBX1 (NP_002576) produced in HEK293T cells, as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PBX1 protein (red) or empty vector (blue) stained using PBX1 antibody)

Product Categories/Family for anti-PBX1 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 11120612
• NCBI GeneID: 5087
• Molecular Weight: 46,265 Da
• NCBI Official Full Name: PBX1
• NCBI Official Synonym Full Names: PBX homeobox 1
• NCBI Official Symbol: PBX1
• NCBI Protein Information: pre-B-cell leukemia transcription factor 1
• UniProt Protein Name: Pre-B-cell leukemia transcription factor 1
• Protein Family: Pre-B-cell leukemia transcription factor
• UniProt Gene Name: PBX1
• UniProt Synonym Gene Names: PRL

NCBI Description

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Probably in complex with MEIS2, is involved in transcriptional regulation by KLF4. Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B. Together with NKX2-5, it is required for spleen development through a mechanism that involves CDKN2B repression ().