IL13 active protein

IL13 protein (Mouse)

• Gene Names: IL13; P600; IL-13
• Applications: Western Blot
• Purity: > 98% pure; IL13 protein was purified by affinity chromatography.
• Synonyms: IL13; IL13 protein (Mouse); IL-13 protein; Interleukin-13 protein; IL13 protein; IL 13 protein; IL 13; IL-13; IL13 active protein

• Host: Mouse
• Specificity: Mouse
• Purity/Purification: > 98% pure; IL13 protein was purified by affinity chromatography.
• Form/Format: Supplied as a sterile liquid with 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, and 1.0% BSA. 0.22 um filtered.
• Concentration: 500 ug/ml (varies by lot)
• Sequence Length: 81

• Applicable Applications for IL13 active protein: Western Blot (WB)
• Application Notes: It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
• Protein Type: Recombinant
• Biological Significance: Mouse interleukin-13, originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4 this relates to a requirement for IL-4R alpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.
• Bioactivity: Measured by TF?1 cell proliferation assay. The ED50 is 3.8 ng/ml, corresponding to a specific activity of 2.7 x 10^5Units/mg.
• Endotoxin Levels: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
• Expression System: E Coli
• Preparation and Storage: Store at -70 degree C or less.

Related Product Information for IL13 active protein

Purified recombinant Mouse IL13 protein

Product Categories/Family for IL13 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 64654183
• NCBI GeneID: 3596
• Molecular Weight: 11.7 kDa
• NCBI Official Full Name: IL13 protein
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: IL13
• NCBI Official Synonym Symbols: P600; IL-13
• NCBI Protein Information: interleukin-13
• UniProt Protein Name: Interleukin-13
• Protein Family: Interleukin
• UniProt Gene Name: IL13
• UniProt Synonym Gene Names: NC30; IL-13
• UniProt Entry Name: IL13_HUMAN

NCBI Description

This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: protein binding; interleukin-13 receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; microglial cell activation; regulation of proton transport; response to lipopolysaccharide; signal transduction; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; positive regulation of protein secretion; cell-cell signaling; positive regulation of B cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; negative regulation of NAD(P)H oxidase activity; cell motility; positive regulation of macrophage activation

Disease: Asthma, Susceptibility To; Allergic Rhinitis