Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using c-RAF antibody)
Rabbit cRAF Polyclonal Antibody | anti-cRAF antibody
cRAF antibody
IHC: 1:50-1:100
ELISA: 1:1000
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using c-RAF antibody)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using c-RAF antibody)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using c-RAF antibody)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using c-RAF antibody)
NCBI and Uniprot Product Information
Uniprot Description
RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Protein type: Protein kinase, Ser/Thr (non-receptor); Oncoprotein; EC 2.7.11.1; Kinase, protein; Protein kinase, TKL; TKL group; RAF family
Cellular Component: Golgi apparatus; mitochondrion; membrane; cytoplasm; plasma membrane; pseudopodium; nucleus; cytosol
Molecular Function: identical protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; nucleotide binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; Ras GTPase binding; MAP kinase kinase kinase activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; receptor signaling protein activity; kinase activity; ATP binding
Biological Process: activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; MAPKKK cascade; intermediate filament cytoskeleton organization and biogenesis; signal transduction; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; negative regulation of cell proliferation; response to hypoxia; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; phosphorylation; negative regulation of protein complex assembly; negative regulation of apoptosis
Research Articles on cRAF
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Product Notes
The cRAF raf1 (Catalog #AAA5300596) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's cRAF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the cRAF raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cRAF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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