Western Blot (WB)
(Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using TNFRSF5 (Phospho-Thr254) antibody)
Rabbit anti-Mouse TNFRSF5 Polyclonal Antibody | anti-TNFRSF5 antibody
TNFRSF5 antibody (Thr254)
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
TNFRSF5 antibody was purified by affinity chromatography
Synonyms
TNFRSF5; Polyclonal Antibody; TNFRSF5 antibody (Thr254); Polyclonal TNFRSF5; Anti-TNFRSF5; MGC:45012; TNF receptor-associated factor 2; TRAF2; TRAP; TRAP3; tumor necrosis factor type 2 receptor associated protein 3; Tumor necrosis factor type 2 receptor-associated protein 3; anti-TNFRSF5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TNFRSF5 antibody (Thr254) detects endogenous levels of TNFRSF5 only when phosphorylated at threonine 254. The antibody will detect species specific phosphorylation as follows: Human: Thr254, Mouse: Thr255
Purity/Purification
TNFRSF5 antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-TNFRSF5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:1000
ELISA: 1:1000
Biological Significance
TNFRSF5 regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis.
Cross-Reactivity
Mouse
Immunogen
TNFRSF5 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human TNFRSF5 around the phosphorylation site of threonine 254 (Q-E-TP-L-H) as the immunogen.
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using TNFRSF5 (Phospho-Thr254) antibody)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using TNFRSF5 (Phospho-Thr254) antibody)
Related Product Information for anti-TNFRSF5 antibody
Rabbit Polyclonal TNFRSF5 antibody (Thr254)
Product Categories/Family for anti-TNFRSF5 antibody
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Product Notes
The TNFRSF5 (Catalog #AAA5300191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNFRSF5 antibody (Thr254) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNFRSF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the TNFRSF5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFRSF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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