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Rabbit RBC (red blood cells) Antibody | anti-RBC antibody

Anti-Sheep RBC (red blood cells), (rabbit IgM)

Applications
For complement fixation and complement dependent haemolytic studies using sheep red blood cells as targets.
Synonyms
RBC (red blood cells); Anti-Sheep RBC (red blood cells); (rabbit IgM); HAEMOLYSIN: RABBIT ANTI-SHEEP RED BLOOD CELL STROMA; Rabbit Anti-Sheep Red Blood Cell Stroma- IgM Fraction; anti-RBC antibody
Ordering
For Research Use Only!
Host
Rabbit
Form/Format
1.0 ml in PBS+ 0.02% NaN3
Applicable Applications for anti-RBC antibody
For complement fixation and complement dependent haemolytic studies using sheep red blood cells as targets.
Sterility
This reagent is not sold as sterile but may be sterilized by filtration if necessary. To minimize loss of volume during filtration, dilute to the final working concentration in the appropriate medium before filtration and filter through a 0.22um Millipore filter (or equivalent).
Method for Determining Hemolytic Titre of Haemolysin Anti-Sheep Red Blood Cell Antiserum
1. Wash and pack sheep red blood cells in VBD media. Dilute to 1 % in VBD.
2. Using a 96-well round-bottom microtitre plate, add the antibody to a final
concentration of 1:2 and dilute serially to a dilution of 1:2 million using doubling dilutions.
3. Add cells and incubate for 30 minutes at room temperature.
4. Centrifuge to pellet the cells and discard the supernatant.
5. Resuspend to the original volume in MBSs Hemo-Lo Guinea Pig Complement diluted to 1:20 in VBD.
6. Incubate for 60 minutes at 37°C.
7. Read plate by visual inspection to determine hemolytic titre.
Immunization
Rabbit anti-sheep red blood cell stroma
Haemolytic
For haemolytic assays using 1-2% srbc's
Properties
The suggested dilution is 1:400.
For complement fixation the appropiate dilution should be determined in house.
Preparation and Storage
Stable at 4°C. For long term storage, aliquot and freeze unused portions at -20°C in volumes appropritate for single usage. Avoid repeated freeze/thaw cycles.
Related Product Information for anti-RBC antibody
Haemolysin is a serum containing a high level of antibody against sheep erythrocytes. When this antiserum is combined with erythrocytes in suspension, the erythrocytes are said to be sensitized (ie. in the presence of free complement they will lyse). A euglobin precipitation was performed on the antiserum in order to purity the IgM fraction.

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Product Notes

The RBC (Catalog #AAA524107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RBC (red blood cells) can be used in a range of immunoassay formats including, but not limited to, For complement fixation and complement dependent haemolytic studies using sheep red blood cells as targets. Researchers should empirically determine the suitability of the RBC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBC (red blood cells), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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