Mouse anti-Human Platelet Factor 4 Monoclonal Antibody | anti-PF4V1 antibody

Anti-Human Platelet Factor 4 (requires heparin for binding),Purified (Clone: KKO) (Mouse IgG2b)

Cat. Num. AAA520552

• Gene Names: PF4V1; PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: Platelet Factor 4; Monoclonal Antibody; Anti-Human Platelet Factor 4 (requires heparin for binding); Purified (Clone: KKO) (Mouse IgG2b); Platelet Factor 4 (requires heparin for binding); Anti-Human Platelet Factor IV/Heparin; anti-PF4V1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG_2bκ
• Clone Number: KKO
• Specificity: This antibody is specific for Human Platelet Factor IV /heparin complexes.
• Form/Format: Purified; IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography). For maximum recovery of contents, spin down tube before use.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 104

• Applicable Applications for anti-PF4V1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: This antibody is suitable for use in ELISA and Western Blot. In ELISA use at a concentration of 0.08ug/ml following coating the plate with PF4/heparin complexes. This can be done by incubating PF4 with an excess of heparin (100-200 U/ml), while coating the plate.
• Immunogen: Human Factor IV purified from the supernatant of thrombin activated platelets.
• Presentation: Biotin: Biotin conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.; HRPO: HRPO conjugated IgG buffered in PBS with 40% glycerol (v/v) and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL. No NaN3 with other preservatives.
• Preparation and Storage: For all formats, store at 4°C. For long term storage (Purified, Biotin, HRPO), aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Related Product Information for anti-PF4V1 antibody

Description: Platelet factor IV is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.

Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FcgammaIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation. (clone KKO), unlike (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.

NCBI and Uniprot Product Information

• NCBI GI #: 292390
• NCBI GeneID: 5197
• UniProt Accession #: P10720; A1L4S0
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4 variant 1
• NCBI Official Symbol: PF4V1
• NCBI Official Synonym Symbols: PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1
• NCBI Protein Information: platelet factor 4 variant; PF4alt; PF4var1; C-X-C motif chemokine 4; platelet factor 4, variant 1 (PF4-like)
• UniProt Protein Name: Platelet factor 4 variant
• Protein Family: Platelet factor
• UniProt Gene Name: PF4V1
• UniProt Synonym Gene Names: CXCL4V1; SCYB4V1
• UniProt Entry Name: PF4V_HUMAN

Uniprot Description

Function: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Ref.5

Subunit structure: Homotetramer. Ref.6

Subcellular location: Secreted.

Post-translational modification: The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).

Miscellaneous: Binding to heparin is much weaker than in the close homolog PF4/CXCL4.

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Mass spectrometry: Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant. Ref.5Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74). Ref.5Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74). Ref.5Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74). Ref.5