LYN A Recombinant Protein | LYNA recombinant protein

LYN A, Active

Cat. Num. AAA517166

• Applications: Western Blot
• Synonyms: LYN A; Active; JTK8; FLJ26625; LYNA recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for LYNA recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for LYNA recombinant protein

Recombinant full-length human LYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_002350.
LYN A is a member of the Src-family of protein kinases that participate in signaling pathways of a variety of cell surface receptors and are localized to the cytoplasmic side of the plasma membrane (1). LYN A is biosynthetically transported to the plasma membrane via the Golgi pool of caveolin along the secretory pathway. The trafficking of LYN A from the Golgi apparatus to the plasma membrane is inhibited by deletion of the kinase domain but not by kinase inactivation. The kinase domain of LYN A plays a role in LYN A trafficking besides catalysis of substrate phosphorylation. cDNA microarray analysis revealed that LYN A may be involved in the changes that are related to tumor pathogenesis (2).

Product Categories/Family for LYNA recombinant protein

Protein Kinases; AKT/PKB Pathway; Cancer; Cardiovascular Disease; Cellular Stress; Cytoplasmic Tyrosine Kinases; Inflammation

References

1. Kasahara, K. et al: Trafficking of Lyn through the Golgi caveolin involves the charged residues on alphaE and alphaI helices in the kinase domain. J. Cell Biol., 2004;165(5):641-52.
2. Baran, C.P. et al: The inositol 5'-phosphatase SHIP-1 and the Src kinase Lyn negatively regulate macrophage colony stimulating factor-induced Akt activity. J. Biol. Chem. 2003; 278(40):38628-36

NCBI and Uniprot Product Information

• NCBI GenBank Nucleotide #: NM_002350
• Molecular Weight: ~81kDa