Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
FAM20C recombinant protein
FAM20C, Active
Applications
Western Blot
Synonyms
FAM20C; Active; GEF-CK; DMP4; G-CK; RNS; FAM20C recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for FAM20C recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for FAM20C recombinant protein
Recombinant human FAM20C (23-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC040074.
FAM20C is Golgi casein kinase that phosphorylates secretory proteins within a Serx-Glu/pSer motif. It phosphorylates caseins and several other secretory proteins including AMTN, ENAM, SPP1 and the small integrin-binding ligand N-linked glycoproteins that are involved in biomineralization (1). FAM20C null mice develop hypophosphatemic rickets along with significant downregulation of osteoblast differentiation markers (2). Mutations in FAM20C gene are associated with Raine syndrome, an osteosclerotic bone dysplasia in humans (3). Additionally, FAM20C is implicated in other physiological processes, such as wound healing, regeneration, and cell migration (4).
FAM20C is Golgi casein kinase that phosphorylates secretory proteins within a Serx-Glu/pSer motif. It phosphorylates caseins and several other secretory proteins including AMTN, ENAM, SPP1 and the small integrin-binding ligand N-linked glycoproteins that are involved in biomineralization (1). FAM20C null mice develop hypophosphatemic rickets along with significant downregulation of osteoblast differentiation markers (2). Mutations in FAM20C gene are associated with Raine syndrome, an osteosclerotic bone dysplasia in humans (3). Additionally, FAM20C is implicated in other physiological processes, such as wound healing, regeneration, and cell migration (4).
Product Categories/Family for FAM20C recombinant protein
References
1. Cui, J. et al. (2015) A secretory kinase complex regulates extracellular protein phosphorylation. eLife. 4:e0612.
2. Wang X, et al: (2012) Inactivation of a novel FGF23 regulator, FAM20C, leads to hypophosphatemic rickets in mice. PLoS Genet. 8(5):e1002708.
3. Tagliabracci VS, et al: (2012) Secreted kinase phosphorylates extracellular proteins that regulate biomineralization. Science. 336(6085):1150-1153
4. Tagliabracci VS, et al: (2015) A single kinase generates the majority of the secreted phosphoproteome. Cell 161: 1619-1632.
2. Wang X, et al: (2012) Inactivation of a novel FGF23 regulator, FAM20C, leads to hypophosphatemic rickets in mice. PLoS Genet. 8(5):e1002708.
3. Tagliabracci VS, et al: (2012) Secreted kinase phosphorylates extracellular proteins that regulate biomineralization. Science. 336(6085):1150-1153
4. Tagliabracci VS, et al: (2015) A single kinase generates the majority of the secreted phosphoproteome. Cell 161: 1619-1632.
NCBI and Uniprot Product Information
Molecular Weight
93kDa
Protein Family
Similar Products
Product Notes
The FAM20C (Catalog #AAA516994) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FAM20C can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the FAM20C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM20C, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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