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Testing Data

DNMT3A recombinant protein

DNMT3A, Active

Gene Names
DNMT3A; TBRS; DNMT3A2; M.HsaIIIA
Applications
Methyltransferase Assay
Synonyms
DNMT3A; Active; DNMT3A recombinant protein
Ordering
For Research Use Only!
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full Length
Sequence Length
4294
Applicable Applications for DNMT3A recombinant protein
Methyltransferase Assay
Species
Human
Tag
GST tag
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data

Testing Data #2

Testing Data #2
Related Product Information for DNMT3A recombinant protein
Description: Recombinant full-length human DNMT3A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: DNMT3A: DNA (cytosine-5-)-methyltransferase 3 alpha plays important role in genomic imprinting and X chromosome inactivation and is essential for mammalian development. DNMT3A appears to function as a de novo methyltrabsferase, since it can methylate unmethylated and hemimethylated DNA with equal efficiencies (1). DNMT3A is required for genome wide de novo methylation and is essential for mammalian development (2). DNMT3A is critical for maintaining active chromatin states of genes that are essential for development.
Product Categories/Family for DNMT3A recombinant protein
References
1. Yanagisawa, Y. et al: The human DNA methyltransferases DNMT3A and DNMT3B have two types of promoters with different CpG contents. Biochim. Biophys. Acta 1577: 457-465, 2002. 2. Okano, M. et al: DNA methyltransferase Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99: 247-257, 1999.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
~162 kDa
NCBI Official Full Name
Homo sapiens DNA (cytosine-5-)-methyltransferase 3 alpha, mRNA
NCBI Official Synonym Full Names
DNA methyltransferase 3 alpha
NCBI Official Symbol
DNMT3A
NCBI Official Synonym Symbols
TBRS; DNMT3A2; M.HsaIIIA
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3A
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 3A
Protein Family
DNA
UniProt Gene Name
DNMT3A
UniProt Synonym Gene Names
Dnmt3a; DNA MTase HsaIIIA; M.HsaIIIA

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]

Uniprot Description

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.37; Methyltransferase; Methyltransferase, DNA

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytoplasm; euchromatin; nuclear matrix; nucleoplasm; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; identical protein binding; protein binding

Biological Process: DNA methylation; genetic imprinting; negative regulation of gene expression, epigenetic

Disease: Tatton-brown-rahman Syndrome

Research Articles on DNMT3A

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Product Notes

The DNMT3A dnmt3a (Catalog #AAA515490) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length. AAA Biotech's DNMT3A can be used in a range of immunoassay formats including, but not limited to, Methyltransferase Assay. Researchers should empirically determine the suitability of the DNMT3A dnmt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT3A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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