SDS-PAGE
UBE2D1 (UBCH5A) recombinant protein
UBE2D1 (UBCH5A) recombinant protein
SDS-PAGE
SDS-PAGE
Scientific Background: UBE2D1 (UBCH5A) or ubiquitin-conjugating enzyme E2D1 is a member of the E2 ubiquitin-conjugating enzyme family which is closely related to a stimulator of iron transport (SFT). UBE2D1 is significantly upregulated in livers of iron-overloaded patients with hereditary hemochromatosis (1). UBE2D1 also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF-1ï¡ by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases (2).
2. Scheffner, M. et.al: Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Proc. Nat. Acad. Sci. 91: 8797-8801, 1994.
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Uniprot Description
Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. Ref.12 Ref.14 Ref.15 Ref.16 Ref.17
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway: Protein modification; protein ubiquitination.
Subunit structure: Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts with RNF11. Ref.10 Ref.11
Subcellular location: Cytoplasm Ref.13.
Tissue specificity: Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis. Ref.2 Ref.8
Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
Caution: Ref.8 cloned and sequenced SFT which consisted of UBE2D1 last coding exon along with intronic sequences on the 5'-end of this exon. A function in iron transport has been described.
Research Articles on UBE2D1 (UBCH5A)
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Product Notes
The UBE2D1 (UBCH5A) ube2d1 (Catalog #AAA515088) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||E.coli!!Source Note||Recombinant full length human UBE2D1 (UBCH5A) was expressed in E. coli cells. AAA Biotech's UBE2D1 (UBCH5A) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the UBE2D1 (UBCH5A) ube2d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2D1 (UBCH5A), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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